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  1. Bug Repository
  2. BUG-187

probe sets not being displayed correctly

    Details

    • Type: Bug
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Fixed
    • Labels:
      None
    • Environment:

      IGB 6.7 (trunk) but also affects 6.6

      Description

      As always, feel free to re-assign this as needed.

      In the trunk version of IGB the Affy probe sets are not displayed correctly.

      To repeat:

      1. choose A_thaliana_Jun_2009
      2. zoom in on a gene region
      3. choose data set IGB (DAS2)>affy>ATH1-121501

      note there probe sets data sets for mouse, human, etc
      you don't have to use A thaliana

      4. load data

      What's happening is that the probe sets are somehow getting put into a different track.

      What you should see is the attached, a screen capture from IGB 6.5.3, following setting the label field to "id."

      (I am showing the id in the image so that you can more easily find this probe set again if you need to.)

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            Hide
            ann.loraine Ann Loraine added a comment -

            The pink bars are the probes. These are getting read from the link.psl file. Note that IGB translates probe-to-target coordinates into genomic coordinates using the genometry data models.

            Show
            ann.loraine Ann Loraine added a comment - The pink bars are the probes. These are getting read from the link.psl file. Note that IGB translates probe-to-target coordinates into genomic coordinates using the genometry data models.
            Hide
            ann.loraine Ann Loraine added a comment -

            Note that pink bars (probes) for minus strand probe sets appear on the bottom of the blue boxes.

            Show
            ann.loraine Ann Loraine added a comment - Note that pink bars (probes) for minus strand probe sets appear on the bottom of the blue boxes.
            Hide
            ann.loraine Ann Loraine added a comment -

            View from IGB 6.5.3.

            Show
            ann.loraine Ann Loraine added a comment - View from IGB 6.5.3.
            Hide
            ann.loraine Ann Loraine added a comment -

            Also, see this Web page for description of link.psl format:

            http://www.affymetrix.com/support/technical/manual/alignments_psl_manual.affx

            Show
            ann.loraine Ann Loraine added a comment - Also, see this Web page for description of link.psl format: http://www.affymetrix.com/support/technical/manual/alignments_psl_manual.affx
            Hide
            ann.loraine Ann Loraine added a comment -

            I found a copy of a link.psl file for ATH1 array here:

            http://www.affymetrix.com/analysis/downloads/na21/ivt/ATH1-121501.ath1.link.psl.zip

            Show
            ann.loraine Ann Loraine added a comment - I found a copy of a link.psl file for ATH1 array here: http://www.affymetrix.com/analysis/downloads/na21/ivt/ATH1-121501.ath1.link.psl.zip
            Hide
            alyssa Alyssa Gulledge (Inactive) added a comment -

            The linked psl file shows up properly in 6.6. None of the files in the 'affy' folders which are labeled as 'probes' (see Homo sapiens or Rattus norveticus) show the pink bars though.

            Show
            alyssa Alyssa Gulledge (Inactive) added a comment - The linked psl file shows up properly in 6.6. None of the files in the 'affy' folders which are labeled as 'probes' (see Homo sapiens or Rattus norveticus) show the pink bars though.
            Hide
            ann.loraine Ann Loraine added a comment -

            Do you mean under the data source NetAffx?

            To test the probe sets visualization for mouse or human, you need to access the NetAffx DAS source, which does not appear to be available at the moment.

            Show
            ann.loraine Ann Loraine added a comment - Do you mean under the data source NetAffx? To test the probe sets visualization for mouse or human, you need to access the NetAffx DAS source, which does not appear to be available at the moment.
            Hide
            ann.loraine Ann Loraine added a comment - - edited

            Opening and viewing the link.psl file worked fine for me.

            Is there any reason to expect that it would not work if coming from a DAS2 server?

            I ask because the NetAffx DAS source is currently down - at least for me.

            Show
            ann.loraine Ann Loraine added a comment - - edited Opening and viewing the link.psl file worked fine for me. Is there any reason to expect that it would not work if coming from a DAS2 server? I ask because the NetAffx DAS source is currently down - at least for me.
            Hide
            hiralv Hiral Vora (Inactive) added a comment -

            Fixed. Works with psl from DAS2 too.

            Show
            hiralv Hiral Vora (Inactive) added a comment - Fixed. Works with psl from DAS2 too.
            Hide
            alyssa Alyssa Gulledge (Inactive) added a comment -

            The tracks are showing in the main image correctly, with the pink probes overlaying the gene models BUT... there are now 2 tracks in the track table, and they behave oddly...

            the track that shows up as 'empty' (ie no load icon, no load mode, no data set, no delete) is the track that controls the image that is seen (ie color changes in this track are seen; yellow in the attached picture). The track in which all of the values are present is the track that is empty (dark blue in attached picture).

            Another issue is that if you 'remove data from selected tracks' on the probe sets, they never re-load, even if you move to a new area of the same chromosome.

            Show
            alyssa Alyssa Gulledge (Inactive) added a comment - The tracks are showing in the main image correctly, with the pink probes overlaying the gene models BUT... there are now 2 tracks in the track table, and they behave oddly... the track that shows up as 'empty' (ie no load icon, no load mode, no data set, no delete) is the track that controls the image that is seen (ie color changes in this track are seen; yellow in the attached picture). The track in which all of the values are present is the track that is empty (dark blue in attached picture). Another issue is that if you 'remove data from selected tracks' on the probe sets, they never re-load, even if you move to a new area of the same chromosome.
            Hide
            alyssa Alyssa Gulledge (Inactive) added a comment -

            Multi track bugs with these files

            Show
            alyssa Alyssa Gulledge (Inactive) added a comment - Multi track bugs with these files
            Hide
            alyssa Alyssa Gulledge (Inactive) added a comment -

            Multi track issue

            Show
            alyssa Alyssa Gulledge (Inactive) added a comment - Multi track issue
            Hide
            hiralv Hiral Vora (Inactive) added a comment -

            Done in r9540.

            Please test it with other probeset files too.

            Show
            hiralv Hiral Vora (Inactive) added a comment - Done in r9540. Please test it with other probeset files too.
            Hide
            alyssa Alyssa Gulledge (Inactive) added a comment -

            There is still a bug/some 'weirdness' with these files. When you check mark them to get them into the data management table, the show up with 2 track checked. If I uncheck 2 track before loading, they still load up as 2 track, with the two track box suddenly rechecked. Then I uncheck and it makes 1 track. However, the pink probes are NOT properly offset to the top/bottom indicating 'side'. They completely overlap the cyan glyph. I have to activate 2 track again and then uncheck it to get them to show the offset.

            If the track is permitted to load as two track and then made 1 track, it loads and displays fine.

            However, both human and plant are properly loading a 'single' track for each probe file.

            Show
            alyssa Alyssa Gulledge (Inactive) added a comment - There is still a bug/some 'weirdness' with these files. When you check mark them to get them into the data management table, the show up with 2 track checked. If I uncheck 2 track before loading, they still load up as 2 track, with the two track box suddenly rechecked. Then I uncheck and it makes 1 track. However, the pink probes are NOT properly offset to the top/bottom indicating 'side'. They completely overlap the cyan glyph. I have to activate 2 track again and then uncheck it to get them to show the offset. If the track is permitted to load as two track and then made 1 track, it loads and displays fine. However, both human and plant are properly loading a 'single' track for each probe file.
            Hide
            hiralv Hiral Vora (Inactive) added a comment -

            Did this happened again?

            Show
            hiralv Hiral Vora (Inactive) added a comment - Did this happened again?
            Hide
            richard Richard Linchangco (Inactive) added a comment -

            Passed V.13601, Mac, Java 1.06.0_37

            Tested with A. thaliana and H. sapiens. Probes appear in pink, offset from the read but in the same track, as depicted in last two photos attached to this bug.

            Show
            richard Richard Linchangco (Inactive) added a comment - Passed V.13601, Mac, Java 1.06.0_37 Tested with A. thaliana and H. sapiens. Probes appear in pink, offset from the read but in the same track, as depicted in last two photos attached to this bug.

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              • Assignee:
                Unassigned
                Reporter:
                ann.loraine Ann Loraine
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                Dates

                • Created:
                  Updated:
                  Resolved: