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  1. User Support
  2. HELP-110

[Biostars] Can IGB load BED 6+3 files?

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    • Type: Support
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
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      Description

      The Biostars post can be found here:

      https://www.biostars.org/p/139264/

      User paedugar wrote Question: Can IGB load BED 6+3 files?:

      Is it possible to load the "broad peak" output files of macs2 (BED 6+3) into the Integrated Genome Browser (IGB)?
      I have tried with no luck, I'm not quite sure whether I need to specify the input format.

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            mason Mason Meyer (Inactive) added a comment -

            User Ann wrote Answer: Can IGB load BED 6+3 files?:

            We have a pending request to support these two formats without the need for manipulating files, and so now probably would be a good time to move ahead with implementing this. When it's ready, we'll post back here to let you know.

            Regards,

            Ann

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            mason Mason Meyer (Inactive) added a comment - User Ann wrote Answer: Can IGB load BED 6+3 files?: We have a pending request to support these two formats without the need for manipulating files, and so now probably would be a good time to move ahead with implementing this. When it's ready, we'll post back here to let you know. Regards, Ann
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            mason Mason Meyer (Inactive) added a comment -

            User pgarcia commented:

            That sounds easy I'll try it out. Thanks!

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            mason Mason Meyer (Inactive) added a comment - User pgarcia commented: That sounds easy I'll try it out. Thanks!
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            mason Mason Meyer (Inactive) added a comment - - edited

            User Nowlan Freese wrote Answer: Can IGB load BED 6+3 files?:

            Hi pgarcia,

            The .broadPeak format is not a currently supported file format in IGB, hover it can easily be converted to the more common .bed format, which is supported.

            To convert the file to a bed file, limit the data to the first 6 columns. To do this on the command line use:
            $cut –f1-6 FILE.narrowPeak > NEWFILE.bed

            Optionally, you can replace the score field (column 5) with any one of the latter fields (columns 7-9 from broad peak format) to make that value appear as the score.
            For more information about bed format: https://genome.ucsc.edu/FAQ/FAQformat.html#format1
            For more information about broad peak format: http://genome.ucsc.edu/FAQ/FAQformat.html#format12

            These file formats (both broadPeak and narrowPeak) have been recently requested by users, and we are preparing to add support for them in the near future.

            Nowlan

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            mason Mason Meyer (Inactive) added a comment - - edited User Nowlan Freese wrote Answer: Can IGB load BED 6+3 files?: Hi pgarcia, The .broadPeak format is not a currently supported file format in IGB, hover it can easily be converted to the more common .bed format, which is supported. To convert the file to a bed file, limit the data to the first 6 columns. To do this on the command line use: $cut –f1-6 FILE.narrowPeak > NEWFILE.bed Optionally, you can replace the score field (column 5) with any one of the latter fields (columns 7-9 from broad peak format) to make that value appear as the score. For more information about bed format: https://genome.ucsc.edu/FAQ/FAQformat.html#format1 For more information about broad peak format: http://genome.ucsc.edu/FAQ/FAQformat.html#format12 These file formats (both broadPeak and narrowPeak) have been recently requested by users, and we are preparing to add support for them in the near future. Nowlan

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                mason Mason Meyer (Inactive)
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