On 2022-01-06, Gloria Muday wrote the following to Ann & Rob, cc-ing Devin Smith (desmith@wfu.edu);

Rob and Ann,

Happy New Year!

I hope you are staying well. I saw that UNC Charlotte's classes are remote for most of January. I hope that does not complicate things too much and keeps everyone safe on your campus.

Devin and I have one question that we need an answer to to continue identifying DE genes from the "are" RNA Seq dataset. Some of the adjusted pvalues came out as NA. We don't understand why that is and what it means. Can you all explain that to use? We prefer to use the adjusted pvalues to filter the dataset, but cannot with some values (with low pvalues) showing up as NA.

Thanks,
Gloria
–
Gloria Muday
Director, Center for Molecular Signaling
Charles M. Allen, Professor of Biology
Wake Forest University
336-758-5316

Loraine replied:

Sure, we can look into it!

Rob, can you send me the script you used to create the data sheet – or (even better!) add it to the repository?

For this task, let's examine the data file output by Rob's code to determine the meaning of the NA values.

Also, we should put the code into the project repository.