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  1. IGB
  2. IGBF-3172

Tabulate splicing support by running arabitag algorithm on junction and bam files

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
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      Description

      Run the arabitag algorithm to tabulate splice junction support using output from find_junctions.sh in linked issue.

      Ref: See diagram from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589529/figure/pld3136-fig-0001/
      See paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589529/

      Rice paper repository: https://bitbucket.org/lorainelab/ricealtsplice/src/master/

      Arabitag algorithm repository: https://bitbucket.org/lorainelab/altspliceanalysis/src/master/

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            ann.loraine Ann Loraine added a comment -

            Thanks [~molly]. I decided to store all the files in the same folder and will use an index / table of contents type strategy to distinguish them. Moving to Done.

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            ann.loraine Ann Loraine added a comment - Thanks [~molly] . I decided to store all the files in the same folder and will use an index / table of contents type strategy to distinguish them. Moving to Done.
            Hide
            ann.loraine Ann Loraine added a comment -

            Re-opening to include new work to tabulate results and read into R for interactive analysis of results.

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            ann.loraine Ann Loraine added a comment - Re-opening to include new work to tabulate results and read into R for interactive analysis of results.
            Hide
            ann.loraine Ann Loraine added a comment -

            Splicing support output files added as "tar" file to https://bitbucket.org/hotpollen/splicing-analysis as TabulateSplicingSupport/results/2022-09-08-results.tar.

            Show
            ann.loraine Ann Loraine added a comment - Splicing support output files added as "tar" file to https://bitbucket.org/hotpollen/splicing-analysis as TabulateSplicingSupport/results/2022-09-08-results.tar.
            Hide
            ann.loraine Ann Loraine added a comment - - edited

            Creating R code for organizing and sanity-checking results. Working on this fork and branch: https://bitbucket.org/aloraine/splicing-analysis/branch/IGBF-3172

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            ann.loraine Ann Loraine added a comment - - edited Creating R code for organizing and sanity-checking results. Working on this fork and branch: https://bitbucket.org/aloraine/splicing-analysis/branch/IGBF-3172
            Hide
            ann.loraine Ann Loraine added a comment -

            Merged changes into master branch. Created new folder with consolidated results:

            • TabulateSplicingSupport/results/ containing file 2022-09-08-results.txt
            Show
            ann.loraine Ann Loraine added a comment - Merged changes into master branch. Created new folder with consolidated results: TabulateSplicingSupport/results/ containing file 2022-09-08-results.txt

              People

              • Assignee:
                ann.loraine Ann Loraine
                Reporter:
                ann.loraine Ann Loraine
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                • Created:
                  Updated:
                  Resolved: