Uploaded image for project: 'IGB'
  1. IGB
  2. IGBF-3281

Muday-144 DE analysis & Volcano Plots

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      Combining Ticket IGBF-3279 with this ticket IGBF-3281:

      2. I know that pipelines/analysis procedures evolve. Can you look at the analysis of DE genes with temperature within each genotype and make sure we have a final set of data. This is pertinent to compare with number 3! I think we decided that IQR 50 was the best metric
      4. Volcano plots with lines drawn at p values (not adjusted p-values) less than 0.05 and Log fold change (base 2) of 1.0. This would be comparing temperature response within genotypes (so 3 plots) and differences between genotypes at each temperature (so 6 plots).

        Attachments

          Issue Links

            Activity

            Mdavis4290 Molly Davis created issue -
            Mdavis4290 Molly Davis made changes -
            Field Original Value New Value
            Epic Link IGBF-3277 [ 22158 ]
            Mdavis4290 Molly Davis made changes -
            Link This issue blocks IGBF-3280 [ IGBF-3280 ]
            Mdavis4290 Molly Davis made changes -
            Link This issue blocks IGBF-3279 [ IGBF-3279 ]
            Mdavis4290 Molly Davis made changes -
            Link This issue blocks IGBF-3278 [ IGBF-3278 ]
            Mdavis4290 Molly Davis made changes -
            Link This issue is blocked by IGBF-3282 [ IGBF-3282 ]
            Mdavis4290 Molly Davis made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            ann.loraine Ann Loraine made changes -
            Sprint Spring 4 2023 Feb 21 [ 164 ] Spring 4 2023 Feb 21, Spring 5 2023 Mar 6 [ 164, 165 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            Show
            Mdavis4290 Molly Davis added a comment - 3-D Volcano Plots: https://cran.r-project.org/web/packages/volcano3D/vignettes/Vignette.html
            Show
            ann.loraine Ann Loraine added a comment - vignette: http://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html
            Mdavis4290 Molly Davis made changes -
            Status In Progress [ 3 ] To-Do [ 10305 ]
            Mdavis4290 Molly Davis made changes -
            Description 4. Volcano plots with lines drawn at p values (not adjusted p-values) less than 0.05 and Log fold change (base 2) of 1.0. This would be comparing temperature response within genotypes (so 3 plots) and differences between genotypes at each temperature (so 6 plots). Combining Ticket IGBF-3279 with this ticket IGBF-3281:

            2. I know that pipelines/analysis procedures evolve. Can you look at the analysis of DE genes with temperature within each genotype and make sure we have a final set of data. This is pertinent to compare with number 3! I think we decided that IQR 50 was the best metric
            4. Volcano plots with lines drawn at p values (not adjusted p-values) less than 0.05 and Log fold change (base 2) of 1.0. This would be comparing temperature response within genotypes (so 3 plots) and differences between genotypes at each temperature (so 6 plots).
            Mdavis4290 Molly Davis made changes -
            Summary Muday-144 Volcano Plots Muday-144 DE analysis & Volcano Plots
            Show
            Mdavis4290 Molly Davis added a comment - Updated Bitbucket Repository: https://bitbucket.org/mdavis4290/analysis-flavonoid-rnaseq/src/main/ Commit: https://bitbucket.org/mdavis4290/analysis-flavonoid-rnaseq/commits/67b6a52222e71dd5dfd4f721c82cf8b88ebd4769
            Mdavis4290 Molly Davis made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            Hide
            ann.loraine Ann Loraine added a comment -

            Merged changes into main branch of team repository and then added 3 new commits to address comments on above commit. Moving to Done.

            Show
            ann.loraine Ann Loraine added a comment - Merged changes into main branch of team repository and then added 3 new commits to address comments on above commit. Moving to Done.
            ann.loraine Ann Loraine made changes -
            Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
            ann.loraine Ann Loraine made changes -
            Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
            ann.loraine Ann Loraine made changes -
            Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
            ann.loraine Ann Loraine made changes -
            Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
            ann.loraine Ann Loraine made changes -
            Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
            ann.loraine Ann Loraine made changes -
            Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
            ann.loraine Ann Loraine made changes -
            Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
            ann.loraine Ann Loraine made changes -
            Resolution Done [ 10000 ]
            Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]

              People

              • Assignee:
                Mdavis4290 Molly Davis
                Reporter:
                Mdavis4290 Molly Davis
              • Votes:
                0 Vote for this issue
                Watchers:
                2 Start watching this issue

                Dates

                • Created:
                  Updated:
                  Resolved: