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  1. IGB
  2. IGBF-3500

Re-run mark-2022-timeseries data with data downloaded from SRA

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      Re-run mark 2022 timeseries data with the name SRP441343 from SRA for both SL4 and SL5 genomes.

      For this task, we need to confirm and sanity-check the mark 2022 time series data that Rob uploaded and submitted to the Sequence Read Archive.

      If the data are good, we will replace all the existing BAM, junctions, etc. files deployed in the "hotpollen" quickload site with newly processed data.
      For this task:

      • Check SRP on NCBI and review submission
      • Download the data onto the cluster by using the SRP name
      • Run nf-core/rnaseq pipeline
      • Run our coverage graph and junctions scripts on the data

      Note that all files should now use their "SRR" names instead of the existing file names.

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          Issue Links

            Activity

            Mdavis4290 Molly Davis created issue -
            Mdavis4290 Molly Davis made changes -
            Field Original Value New Value
            Epic Link IGBF-2993 [ 21429 ]
            Mdavis4290 Molly Davis made changes -
            Link This issue relates to IGBF-3406 [ IGBF-3406 ]
            Mdavis4290 Molly Davis made changes -
            Link This issue relates to IGBF-3499 [ IGBF-3499 ]
            Mdavis4290 Molly Davis made changes -
            Rank Ranked higher
            Mdavis4290 Molly Davis made changes -
            Assignee Molly Davis [ molly ]
            Mdavis4290 Molly Davis made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            Mdavis4290 Molly Davis made changes -
            Description SRP441343 Re-run mark 2022 timeseries data with the name SRP441343 from SRA for both SL4 and SL5 genomes.
            ann.loraine Ann Loraine made changes -
            Sprint Fall 6 [ 182 ] Fall 6, Fall 7 [ 182, 183 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            Mdavis4290 Molly Davis made changes -
            Assignee Molly Davis [ molly ]
            Mdavis4290 Molly Davis made changes -
            Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
            Mdavis4290 Molly Davis made changes -
            Assignee Robert Reid [ robertreid ]
            Mdavis4290 Molly Davis made changes -
            Description Re-run mark 2022 timeseries data with the name SRP441343 from SRA for both SL4 and SL5 genomes. Re-run mark 2022 timeseries data with the name SRP441343 from SRA for both SL4 and SL5 genomes.

            For this task, we need to confirm and sanity-check the mark 2022 time series data that Rob uploaded and submitted to the Sequence Read Archive.

            If the data are good, we will replace all the existing BAM, junctions, etc. files deployed in the "hotpollen" quickload site with newly processed data.
            For this task:
            * Check SRP on NCBI and review submission
            * Download the data onto the cluster by using the SRP name
            * Run nf-core/rnaseq pipeline
            * Run our coverage graph and junctions scripts on the data

            Note that all files should now use their "SRR" names instead of the existing file names.
            Mdavis4290 Molly Davis made changes -
            Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
            robofjoy Robert Reid made changes -
            Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
            robofjoy Robert Reid made changes -
            Assignee Robert Reid [ robertreid ] Molly Davis [ molly ]
            Mdavis4290 Molly Davis made changes -
            Assignee Molly Davis [ molly ]
            Mdavis4290 Molly Davis made changes -
            Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
            ann.loraine Ann Loraine made changes -
            Sprint Fall 6, Fall 7 [ 182, 183 ] Fall 6, Fall 7, Fall 8 [ 182, 183, 184 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            Mdavis4290 Molly Davis made changes -
            Assignee Ann Loraine [ aloraine ]
            ann.loraine Ann Loraine made changes -
            Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
            ann.loraine Ann Loraine made changes -
            Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
            ann.loraine Ann Loraine made changes -
            Assignee Ann Loraine [ aloraine ]
            ann.loraine Ann Loraine made changes -
            Sprint Fall 6, Fall 7, Fall 8 [ 182, 183, 184 ] Fall 6, Fall 7, Spring 2 [ 182, 183, 186 ]
            Mdavis4290 Molly Davis made changes -
            Sprint Fall 6, Fall 7, Spring 2 [ 182, 183, 186 ] Fall 6, Fall 7, Spring 1 [ 182, 183, 185 ]
            Mdavis4290 Molly Davis made changes -
            Assignee Molly Davis [ molly ]
            Mdavis4290 Molly Davis made changes -
            Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
            Mdavis4290 Molly Davis made changes -
            Resolution Done [ 10000 ]
            Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]

              People

              • Assignee:
                Mdavis4290 Molly Davis
                Reporter:
                Mdavis4290 Molly Davis
              • Votes:
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                Watchers:
                3 Start watching this issue

                Dates

                • Created:
                  Updated:
                  Resolved: