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  1. IGB
  2. IGBF-3529

Change Volcano plot colors & X-axis

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      During a meeting Muday lab requested to change the volcano plot colors to a more saturated blue and red. Then the middle would be grey instead of black. They also requested to have all of the x-axis (-10,10) including the custom volcano plots.

      Note: These changes need to be implemented with all of the other FindControlVsStress & MakeCustomVolcanoPlots markdowns. StatFunctions.R will have the color and axis changes in the code.

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            Mdavis4290 Molly Davis created issue -
            Mdavis4290 Molly Davis made changes -
            Field Original Value New Value
            Epic Link IGBF-3446 [ 22548 ]
            Mdavis4290 Molly Davis made changes -
            Link This issue relates to IGBF-3520 [ IGBF-3520 ]
            Mdavis4290 Molly Davis made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            Mdavis4290 Molly Davis made changes -
            Description During a meeting Muday lab requested to change the volcano plot colors to a more saturated blue and red. Then the middle would be grey instead of black. They also requested to hav all of the x-axis (-10,10) including the custom volcano plots. During a meeting Muday lab requested to change the volcano plot colors to a more saturated blue and red. Then the middle would be grey instead of black. They also requested to have all of the x-axis (-10,10) including the custom volcano plots.

            Note: These changes need to be implemented with all of the other FindControlVsStress & MakeCustomVolcanoPlots markdowns. StatFunctions.R will have the color and axis changes in the code.
            Hide
            Mdavis4290 Molly Davis added a comment - - edited

            Branch: https://bitbucket.org/mdavis4290/molly5-flavonoid-rnaseq/branch/IGBF-3529

            Changes: The only things that were changed to the code was the volcano plots colors and the x-axis min and max to (-10,10). The code changes the knitted pdf files because the volcano plots not have different colors and x-axis.

            Show
            Mdavis4290 Molly Davis added a comment - - edited Branch : https://bitbucket.org/mdavis4290/molly5-flavonoid-rnaseq/branch/IGBF-3529 Changes : The only things that were changed to the code was the volcano plots colors and the x-axis min and max to (-10,10). The code changes the knitted pdf files because the volcano plots not have different colors and x-axis.
            Hide
            Mdavis4290 Molly Davis added a comment - - edited

            Reviewer:

            • Pull each file from the branch and make sure that the colors are a more vibrant blue and red and that the x-axis min and max are (-10,10).
            • Make sure no other code was changed and that the volcano plots are visually appealing.
            • Do the plots need larger points? Are the colors vibrant enough? Are the point shapes appropriate on the plots?

            Thank you!

            Robert Reid

            If everything is fine, here is the Pull Request so things can move forward while I am on holiday break!

            https://bitbucket.org/hotpollen/flavonoid-rnaseq/pull-requests/38

            Show
            Mdavis4290 Molly Davis added a comment - - edited Reviewer : Pull each file from the branch and make sure that the colors are a more vibrant blue and red and that the x-axis min and max are (-10,10). Make sure no other code was changed and that the volcano plots are visually appealing. Do the plots need larger points? Are the colors vibrant enough? Are the point shapes appropriate on the plots? Thank you! Robert Reid If everything is fine, here is the Pull Request so things can move forward while I am on holiday break! https://bitbucket.org/hotpollen/flavonoid-rnaseq/pull-requests/38
            Mdavis4290 Molly Davis made changes -
            Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
            Mdavis4290 Molly Davis made changes -
            Assignee Molly Davis [ molly ] Robert Reid [ robertreid ]
            ann.loraine Ann Loraine made changes -
            Assignee Robert Reid [ robertreid ] Ann Loraine [ aloraine ]
            Hide
            ann.loraine Ann Loraine added a comment -

            Pulling branch to my local for review.

            Show
            ann.loraine Ann Loraine added a comment - Pulling branch to my local for review.
            Hide
            ann.loraine Ann Loraine added a comment - - edited

            Files changed include:

            commit cd485e7d2b06be5622954609647eac170df32402 (HEAD -> IGBF-3529, molly/IGBF-3529)
            Author: MollyDSnk <MollyDSnk@users.noreply.github.com>
            Date:   Fri Dec 15 11:09:19 2023 -0500
            
                IGBF-3529 Change Volcano plot colors & X-axis
            
            -       -       72_F3H_PollenTube/CustomVolcanoPlots-SL4-DeSeq2.pdf
            -       -       72_F3H_PollenTube/FindControlVsStressDEGenes-SL4-DESeq2.pdf
            -       -       72_F3H_PollenTube/FindControlVsStressDEGenes-SL4-edgeR.pdf
            -       -       72_F3H_PollenTube/FindControlVsStressDEGenes-SL5-DESeq2.pdf
            -       -       72_F3H_PollenTube/FindControlVsStressDEGenes-SL5-edgeR.pdf
            6       6       72_F3H_PollenTube/MakeCustomVolcanoPlots.Rmd
            -       -       72_F3H_PollenTube/MakeCustomVolcanoPlots.pdf
            7       7       72_F3H_PollenTube/StatFunctions.R
            
            commit 92a394323efb071ef8a881f0f5780d5b38e1ba32 (origin/main, origin/HEAD, molly/main, main)
            Author: aloraine <aloraine@uncc.edu>
            Date:   Wed Nov 22 15:00:04 2023 -0500
            
                IGBF-3504 Group1 should be numerator, group2 denominator
            
            7       6       72_F3H_PollenTube/FindMutantVsWildtypeDEGenes-DESeq2.Rmd
            -       -       72_F3H_PollenTube/FindMutantVsWildtypeDEGenes-DESeq2.pdf
            101943  103055  72_F3H_PollenTube/results/MvW-SL4.txt
            99086   97656   72_F3H_PollenTube/results/MvW-SL5.txt
            

            Molly has changed the PDFs resulting from running differential expression analysis, reflecting the change in plotting function present in StatFunction.R, in which the point colors were changed, as noted above.

            Checking to see if the results file itself changes upon re-running the corresponding .Rmd files.

            Show
            ann.loraine Ann Loraine added a comment - - edited Files changed include: commit cd485e7d2b06be5622954609647eac170df32402 (HEAD -> IGBF-3529, molly/IGBF-3529) Author: MollyDSnk <MollyDSnk@users.noreply.github.com> Date: Fri Dec 15 11:09:19 2023 -0500 IGBF-3529 Change Volcano plot colors & X-axis - - 72_F3H_PollenTube/CustomVolcanoPlots-SL4-DeSeq2.pdf - - 72_F3H_PollenTube/FindControlVsStressDEGenes-SL4-DESeq2.pdf - - 72_F3H_PollenTube/FindControlVsStressDEGenes-SL4-edgeR.pdf - - 72_F3H_PollenTube/FindControlVsStressDEGenes-SL5-DESeq2.pdf - - 72_F3H_PollenTube/FindControlVsStressDEGenes-SL5-edgeR.pdf 6 6 72_F3H_PollenTube/MakeCustomVolcanoPlots.Rmd - - 72_F3H_PollenTube/MakeCustomVolcanoPlots.pdf 7 7 72_F3H_PollenTube/StatFunctions.R commit 92a394323efb071ef8a881f0f5780d5b38e1ba32 (origin/main, origin/HEAD, molly/main, main) Author: aloraine <aloraine@uncc.edu> Date: Wed Nov 22 15:00:04 2023 -0500 IGBF-3504 Group1 should be numerator, group2 denominator 7 6 72_F3H_PollenTube/FindMutantVsWildtypeDEGenes-DESeq2.Rmd - - 72_F3H_PollenTube/FindMutantVsWildtypeDEGenes-DESeq2.pdf 101943 103055 72_F3H_PollenTube/results/MvW-SL4.txt 99086 97656 72_F3H_PollenTube/results/MvW-SL5.txt Molly has changed the PDFs resulting from running differential expression analysis, reflecting the change in plotting function present in StatFunction.R, in which the point colors were changed, as noted above. Checking to see if the results file itself changes upon re-running the corresponding .Rmd files.
            Hide
            ann.loraine Ann Loraine added a comment -

            Re-running the .Rmd files did not change results files, just the knitted Markdown. The .Rmd files include a call to the system "date" function, updating the header of the knitted output PDF file to show the current date.

            All looks good. Proceeding to merge PR from Molly Davis.

            Show
            ann.loraine Ann Loraine added a comment - Re-running the .Rmd files did not change results files, just the knitted Markdown. The .Rmd files include a call to the system "date" function, updating the header of the knitted output PDF file to show the current date. All looks good. Proceeding to merge PR from Molly Davis.
            ann.loraine Ann Loraine made changes -
            Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
            ann.loraine Ann Loraine made changes -
            Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
            ann.loraine Ann Loraine made changes -
            Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
            ann.loraine Ann Loraine made changes -
            Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
            ann.loraine Ann Loraine made changes -
            Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
            ann.loraine Ann Loraine made changes -
            Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
            ann.loraine Ann Loraine made changes -
            Resolution Done [ 10000 ]
            Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]
            ann.loraine Ann Loraine made changes -
            Assignee Ann Loraine [ aloraine ] Molly Davis [ molly ]

              People

              • Assignee:
                Mdavis4290 Molly Davis
                Reporter:
                Mdavis4290 Molly Davis
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                Dates

                • Created:
                  Updated:
                  Resolved: