Uploaded image for project: 'IGB'
  1. IGB
  2. IGBF-3570

Create email draft to update Muday lab of volcano plot ceiling

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      Refer to ticket IGBF-3530.

        Attachments

          Issue Links

            Activity

            Hide
            Mdavis4290 Molly Davis added a comment -

            Hi all,

            I hope everyone had a wonderful holiday season!

            Dr. Reid and I have finished working on the ceiling code for the volcano plots and wanted to send out an update. We made the y-axis max 20 on the volcano plots and any points that had a value higher than 20 were edited to hit the ceiling of the plot. The updated files are the following in the repo:

            If interested, the line of code that made this possible was d$pvalue= ifelse(-log10(d$pvalue) < 20,d$pvalue,1e-20).

            Let us know what you think and if you have any more requests! We were thinking of making the points at the ceiling a different color and/or shape and then making sure there was no overlapping issues for the points.

            Thanks so much!

            Sincerely, Molly

            Show
            Mdavis4290 Molly Davis added a comment - Hi all , I hope everyone had a wonderful holiday season! Dr. Reid and I have finished working on the ceiling code for the volcano plots and wanted to send out an update. We made the y-axis max 20 on the volcano plots and any points that had a value higher than 20 were edited to hit the ceiling of the plot. The updated files are the following in the repo: Plots : https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/CustomVolcanoPlots-SL4-DeSeq2.pdf Knitted markdown : https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/MakeCustomVolcanoPlots.pdf Code : https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/MakeCustomVolcanoPlots.Rmd If interested, the line of code that made this possible was d$pvalue= ifelse(-log10(d$pvalue) < 20,d$pvalue,1e-20) . Let us know what you think and if you have any more requests! We were thinking of making the points at the ceiling a different color and/or shape and then making sure there was no overlapping issues for the points. Thanks so much! Sincerely, Molly
            Hide
            Mdavis4290 Molly Davis added a comment - - edited

            Question: Should I add an explanation of why we wanted to create a ceiling in the first place? If so, please add your thoughts on why we need a ceiling for the volcano plots.

            Show
            Mdavis4290 Molly Davis added a comment - - edited Question : Should I add an explanation of why we wanted to create a ceiling in the first place? If so, please add your thoughts on why we need a ceiling for the volcano plots.
            Hide
            robofjoy Robert Reid added a comment -

            These links look good.

            The email reads well and is straightforward.

            To answer your question, yes. Let's tell them why we were adding the ceiling. Something to the effect of:

            "As a reminder we wanted to add a ceiling to the our volcano plots so that we don't lose any data points when we present a zoomed in volcano plot. And to ensure that the volcano plot still retains its appearance of a volcano, demonstrating the expected distribution of data points."

            Show
            robofjoy Robert Reid added a comment - These links look good. The email reads well and is straightforward. To answer your question, yes. Let's tell them why we were adding the ceiling. Something to the effect of: "As a reminder we wanted to add a ceiling to the our volcano plots so that we don't lose any data points when we present a zoomed in volcano plot. And to ensure that the volcano plot still retains its appearance of a volcano, demonstrating the expected distribution of data points."
            Hide
            robofjoy Robert Reid added a comment -

            Something weird is happening in Jira related to moving the task in the columns.

            So I am just assigning this task back to Molly.

            R

            Show
            robofjoy Robert Reid added a comment - Something weird is happening in Jira related to moving the task in the columns. So I am just assigning this task back to Molly. R
            Hide
            Mdavis4290 Molly Davis added a comment - - edited

            Final Draft:

            Hi all,

            I hope everyone had a wonderful holiday season!

            As a reminder we wanted to add a ceiling to the the volcano plots to present a more volcano shaped plot. To do this we added a ceiling that ensures no points are lost and are just kept at the ceiling if higher than 20 -log10 P. Dr. Reid and I have finished working on the ceiling code for the volcano plots and wanted to send out an update. We made the y-axis max 20 on the volcano plots and any points that had a value higher than 20 were edited to hit the ceiling of the plot. The updated files are the following in the repo:

            If interested, the line of code that made this possible was d$pvalue= ifelse(-log10(d$pvalue) < 20,d$pvalue,1e-20).

            Let us know what you think and if you have any more requests! We were thinking of making the points at the ceiling a different color and/or shape and then making sure there was no overlapping issues for the points.

            Thanks so much!

            Sincerely, Molly

            Needs a final review!

            Show
            Mdavis4290 Molly Davis added a comment - - edited Final Draft : Hi all , I hope everyone had a wonderful holiday season! As a reminder we wanted to add a ceiling to the the volcano plots to present a more volcano shaped plot. To do this we added a ceiling that ensures no points are lost and are just kept at the ceiling if higher than 20 -log10 P. Dr. Reid and I have finished working on the ceiling code for the volcano plots and wanted to send out an update. We made the y-axis max 20 on the volcano plots and any points that had a value higher than 20 were edited to hit the ceiling of the plot. The updated files are the following in the repo: Plots : https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/CustomVolcanoPlots-SL4-DeSeq2.pdf Knitted markdown : https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/MakeCustomVolcanoPlots.pdf Code : https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/MakeCustomVolcanoPlots.Rmd If interested, the line of code that made this possible was d$pvalue= ifelse(-log10(d$pvalue) < 20,d$pvalue,1e-20) . Let us know what you think and if you have any more requests! We were thinking of making the points at the ceiling a different color and/or shape and then making sure there was no overlapping issues for the points. Thanks so much! Sincerely, Molly Needs a final review!
            Hide
            robofjoy Robert Reid added a comment -

            Yes !! This looks great, send it off and move this task to done !!

            Show
            robofjoy Robert Reid added a comment - Yes !! This looks great, send it off and move this task to done !!

              People

              • Assignee:
                Mdavis4290 Molly Davis
                Reporter:
                Mdavis4290 Molly Davis
              • Votes:
                0 Vote for this issue
                Watchers:
                2 Start watching this issue

                Dates

                • Created:
                  Updated:
                  Resolved: