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  1. IGB
  2. IGBF-3638

Make sample sheet for SRP441343 and add to makeAnnotsXml.py

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      mark-2022-timeseries experiment has been submitted to SRA and rerun with nextflow. A sanity check will need to be performed on the original data and the rerun SRA data to ensure the submission to NCBI was correct.

      The next step is adding the SRA data to IGB Quickload. To do this a sample sheet is required.

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          Activity

          Hide
          ann.loraine Ann Loraine added a comment -

          Sample sheet is good. Moving to DONE.

          Show
          ann.loraine Ann Loraine added a comment - Sample sheet is good. Moving to DONE.
          Hide
          ann.loraine Ann Loraine added a comment -

          PR is merged. Ready for testing.

          Show
          ann.loraine Ann Loraine added a comment - PR is merged. Ready for testing.
          Show
          Mdavis4290 Molly Davis added a comment - PR : https://bitbucket.org/hotpollen/genome-browser-visualization/pull-requests/6
          Hide
          ann.loraine Ann Loraine added a comment -

          I did not try to run the new code as Molly Davis has already been able to run it, as noted above.
          Moving to "ready for pull request."

          Show
          ann.loraine Ann Loraine added a comment - I did not try to run the new code as Molly Davis has already been able to run it, as noted above. Moving to "ready for pull request."
          Show
          Mdavis4290 Molly Davis added a comment - Branch : https://bitbucket.org/mdavis4290/molly-genome-browser-visualization/branch/IGBF-3638
          Hide
          Mdavis4290 Molly Davis added a comment - - edited

          Adding SRA submission to makeAnnotsXml.py

              # Johnson lab mark-2022-timeseries SRA submission SRP441343
              repo = "https://bitbucket.org/hotpollen/splicing-analysis"
              branch = "main"
              filename = "SRP441343_sample_sheet.xlsx"
              path = "ExternalData/"+filename
              getSampleSheetFromRepo(filename,repo,path,branch)
              spreadsheet = pd.read_excel(filename)
              sample_sheet = SampleSheet(spreadsheet)
              sample_sheet.addGenome(SL5)
              sample_sheet.addGenome(SL4)
              to_return.append(sample_sheet)
          
          export PYTHONPATH=/Users/mollydavis333/Desktop/igbquickload
          
          python -m pip install requests
          
          pip install openpyxl
          
          conda create -c conda-forge -n name_of_my_env python pandas
          
          source activate name_of_my_env
          
          ./makeAnnotsXml.py
          
          Show
          Mdavis4290 Molly Davis added a comment - - edited Adding SRA submission to makeAnnotsXml.py # Johnson lab mark-2022-timeseries SRA submission SRP441343 repo = "https: //bitbucket.org/hotpollen/splicing-analysis" branch = "main" filename = "SRP441343_sample_sheet.xlsx" path = "ExternalData/" +filename getSampleSheetFromRepo(filename,repo,path,branch) spreadsheet = pd.read_excel(filename) sample_sheet = SampleSheet(spreadsheet) sample_sheet.addGenome(SL5) sample_sheet.addGenome(SL4) to_return.append(sample_sheet) export PYTHONPATH=/Users/mollydavis333/Desktop/igbquickload python -m pip install requests pip install openpyxl conda create -c conda-forge -n name_of_my_env python pandas source activate name_of_my_env ./makeAnnotsXml.py
          Hide
          ann.loraine Ann Loraine added a comment -

          Please check with makeAnnotsXml.py for the next step.

          Show
          ann.loraine Ann Loraine added a comment - Please check with makeAnnotsXml.py for the next step.
          Show
          Mdavis4290 Molly Davis added a comment - - edited Branch : https://bitbucket.org/mdavis4290/molly-2-splicing-analysis/branch/IGBF-3638b

            People

            • Assignee:
              Mdavis4290 Molly Davis
              Reporter:
              Mdavis4290 Molly Davis
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              Dates

              • Created:
                Updated:
                Resolved: