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  1. IGB
  2. IGBF-3687

Document and publicize IGB tardigrade assembly support

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
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      Description

      Tardigrade - water bear - is an emerging model system for studying how animals can survive in extreme environments.

      Although there are a few genome assemblies available for tardigrade species, the assemblies are still very "drafty" - lots of contigs and no chromosome level assemblies.

      The annotations are also very "drafty" - not yet fully validated using RNA-Seq or cDNA sequence data.

      And yet, researchers are proceeding with studying how tardigrade gene expression responds to changes in environmental conditions, such as the presence or absence of ionizing radiation.

      Because IGB offers many features for exploring genome assemblies and annotations that are not yet well-characterized, we could help researchers in these fields a lot by letting them know about IGB and these features.

      For this task, develop demonstration materials explaining why and how IGB can help researchers learning about or working with tardigrade genome resources.

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          Activity

          Hide
          ann.loraine Ann Loraine added a comment -

          what: introduce tardigrade genome resource in IGB; show IGB, show how to find tardigrade (aka water bear); show how to select a gene model and do a blast or interpro search; show how to access RNA-Seq data and assess gene expression visually; or assess sequence differences
          who: tardigrade researchers who want to use RNA-Seq or other genomic technologies in their research; others who are working with emerging model organisms and their draft genome assemblies
          why: answer research questions about tardigrade, like: what genes are up-regulated under stress in tardigrade?

          Show
          ann.loraine Ann Loraine added a comment - what: introduce tardigrade genome resource in IGB; show IGB, show how to find tardigrade (aka water bear); show how to select a gene model and do a blast or interpro search; show how to access RNA-Seq data and assess gene expression visually; or assess sequence differences who: tardigrade researchers who want to use RNA-Seq or other genomic technologies in their research; others who are working with emerging model organisms and their draft genome assemblies why: answer research questions about tardigrade, like: what genes are up-regulated under stress in tardigrade?
          Hide
          ann.loraine Ann Loraine added a comment -

          outline:

          • igb is a free and open source genome browser you install on your computer by visiting bioiviz.org
          • download by clicking on the link and running through the installer wizard
          • once igb is running, you use the current genome tab to select the genome assembly of interest - species is Hypsibius exemplaris and genome assembly version is H_exemplaris_Z151_Apr_2017
          • show how the genome assembly loads and includes many thousands of contigs and the gene models load automatically
          • show how there is a "i" button next to the gene models name; if you click there you will go to a web site we've set up with some information about the data files being shown with a link to the original data we got from NCBI (need to check that the link is there and that it works)
          • mention that in future, we can put RNA-Seq data sets there and mention which data set we are probably going to deploy first
          • then show the advanced search to see if we can find genes that are related to ioniziing radiation mention that tardigrade are famous for being able to withstand ionizing radiation; provide a link to a paper
          • locate a gene in the list of results, double-click on it to zoom in
          • show how you can use blast search and also protannot to better understand the gene function
          • show how you can make a bookmark and make a note about the gene so as to return to it later
          Show
          ann.loraine Ann Loraine added a comment - outline: igb is a free and open source genome browser you install on your computer by visiting bioiviz.org download by clicking on the link and running through the installer wizard once igb is running, you use the current genome tab to select the genome assembly of interest - species is Hypsibius exemplaris and genome assembly version is H_exemplaris_Z151_Apr_2017 show how the genome assembly loads and includes many thousands of contigs and the gene models load automatically show how there is a "i" button next to the gene models name; if you click there you will go to a web site we've set up with some information about the data files being shown with a link to the original data we got from NCBI (need to check that the link is there and that it works) mention that in future, we can put RNA-Seq data sets there and mention which data set we are probably going to deploy first then show the advanced search to see if we can find genes that are related to ioniziing radiation mention that tardigrade are famous for being able to withstand ionizing radiation; provide a link to a paper locate a gene in the list of results, double-click on it to zoom in show how you can use blast search and also protannot to better understand the gene function show how you can make a bookmark and make a note about the gene so as to return to it later
          Hide
          ann.loraine Ann Loraine added a comment -

          This is good enough for now. Moving on to new tasks.

          Show
          ann.loraine Ann Loraine added a comment - This is good enough for now. Moving on to new tasks.

            People

            • Assignee:
              ann.loraine Ann Loraine
              Reporter:
              ann.loraine Ann Loraine
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                Updated:
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