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  1. IGB
  2. IGBF-3708

Download tardigrade RNA-Seq data onto cluster

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      Download the data sets identified in the linked ticket into the "data processing" directory on the cluster.

      Instructions:

      1) For each "SRP" accession (the identifier that uniquely identifies a data set), create a folder with the same name as the dataset. For example, if the dataset accession is SRP123456, make a folder named SRP123456. Make sure the folder is group-writeable so that other people in the group can add files to it. (By default, it will likely already be group-executable and group-readable, but please check it just in case.)

      2) Use the "run selector" tool to make a meta-data file listing all the "runs" (SRR id's) plus library names, sample names, etc. for each "SRP" accession. Add it to the data set's folder.

      3) Download fastq files and save them to a subfolder named "fastq" within each "SRP" folder.

      4) Check that the data downloaded correctly. Talk with Molly and Rob Reid about how best to do that. Also, do some research (google, biostars, etc) for fast and easy ways to check that the download process did not fail. Another possibility: run fastQC on the data and compare the output to the meta-data file from the run selector tool? If you notice any problems, fix them.

      5) Compress all the fastq files using gzip.

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            ann.loraine Ann Loraine created issue -
            ann.loraine Ann Loraine made changes -
            Field Original Value New Value
            Epic Link IGBF-1395 [ 17470 ]
            ann.loraine Ann Loraine made changes -
            Link This issue relates to IGBF-3685 [ IGBF-3685 ]
            pkulzer Paige Kulzer made changes -
            Sprint Spring 8 [ 192 ] Spring 9 [ 193 ]
            pkulzer Paige Kulzer made changes -
            Assignee Paige Kulzer [ pkulzer ]
            pkulzer Paige Kulzer made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            pkulzer Paige Kulzer made changes -
            Link This issue relates to IGBF-3721 [ IGBF-3721 ]
            pkulzer Paige Kulzer made changes -
            Status In Progress [ 3 ] To-Do [ 10305 ]
            ann.loraine Ann Loraine made changes -
            Sprint Spring 9 [ 193 ] Spring 9, Spring 10 [ 193, 194 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            pkulzer Paige Kulzer made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            pkulzer Paige Kulzer made changes -
            Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
            pkulzer Paige Kulzer made changes -
            Assignee Paige Kulzer [ pkulzer ] Ann Loraine [ aloraine ]
            pkulzer Paige Kulzer made changes -
            Link This issue relates to IGBF-3735 [ IGBF-3735 ]
            pkulzer Paige Kulzer made changes -
            Summary Download tardigrade RNA-Seq onto cluster Download tardigrade RNA-Seq data onto cluster
            ann.loraine Ann Loraine made changes -
            Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
            ann.loraine Ann Loraine made changes -
            Status First Level Review in Progress [ 10301 ] Needs 1st Level Review [ 10005 ]
            ann.loraine Ann Loraine made changes -
            Sprint Spring 9, Spring 10 [ 193, 194 ] Spring 9, Spring 10, Summer 1 [ 193, 194, 195 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            ann.loraine Ann Loraine made changes -
            Sprint Spring 9, Spring 10, Summer 1 [ 193, 194, 195 ] Spring 9, Spring 10, Summer 1, Summer 2 [ 193, 194, 195, 196 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            ann.loraine Ann Loraine made changes -
            Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
            ann.loraine Ann Loraine made changes -
            Status First Level Review in Progress [ 10301 ] To-Do [ 10305 ]
            ann.loraine Ann Loraine made changes -
            Sprint Spring 9, Spring 10, Summer 1, Summer 2 [ 193, 194, 195, 196 ] Spring 9, Spring 10, Summer 1, Summer 2, Summer 3 [ 193, 194, 195, 196, 197 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            ann.loraine Ann Loraine made changes -
            Epic Link IGBF-1395 [ 17470 ] IGBF-3778 [ 22997 ]
            ann.loraine Ann Loraine made changes -
            Sprint Spring 9, Spring 10, Summer 1, Summer 2, Summer 3 [ 193, 194, 195, 196, 197 ] Spring 9, Spring 10, Summer 1, Summer 2, Summer 3, Summer 4 [ 193, 194, 195, 196, 197, 198 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            ann.loraine Ann Loraine made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            ann.loraine Ann Loraine made changes -
            Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
            ann.loraine Ann Loraine made changes -
            Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
            ann.loraine Ann Loraine made changes -
            Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
            ann.loraine Ann Loraine made changes -
            Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
            ann.loraine Ann Loraine made changes -
            Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
            ann.loraine Ann Loraine made changes -
            Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
            ann.loraine Ann Loraine made changes -
            Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
            ann.loraine Ann Loraine made changes -
            Resolution Done [ 10000 ]
            Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]

              People

              • Assignee:
                ann.loraine Ann Loraine
                Reporter:
                ann.loraine Ann Loraine
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                Dates

                • Created:
                  Updated:
                  Resolved: