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  1. IGB
  2. IGBF-3713

Checking Mark and Rasha's SRA datasets and exploring proteomics

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      This task is to check the 2 SRA datasets that are related to Rasha's upcoming papers.

      Email from Mark:
      We're working on a paper that will draw on three data sets and we want to make sure we have the SRA submissions all set.

      1. Heinz, Tam, Nag pollen tubes
      @ 30', 60', and 120'
      @ 28 and 34
      This experiment was performed by Rasha

      2. Mature Pollen
      28c and 37c for one hour
      Heinz, Tam, Nag, and Malintka
      This experiment was performed by Michelle Medina

      Do we have a stand alone SRA entries for these experiments? I just want to check to make sure so that we can cite it properly.

      The third data set is proteomics data
      Heinz, Tam, Nag pollen tubes
      @ 30' and 120'
      @ 28 and 34
      Mature Pollen - no treatment (dry pollen)

      This experiment was performed by Rasha. Do you have any advice on a repository for these proteomics data?

      We need to

      1. Check the pollen tube data
      2. Check the mature Pollen data
      3. Make a table that is a subset of the data they used.
      4. Explore if there is an NCBi proteomics data solution.

        Attachments

          Activity

          robofjoy Robert Reid created issue -
          robofjoy Robert Reid made changes -
          Field Original Value New Value
          Epic Link IGBF-2993 [ 21429 ]
          robofjoy Robert Reid made changes -
          Status To-Do [ 10305 ] In Progress [ 3 ]
          Hide
          robofjoy Robert Reid added a comment -

          Part 1:
          Bioproject PRJNA980666

          54 samples: 4 varieties * 3 time points * 2 conditions *3 replicates

          Part 2 Mature Pollen:
          I think this is the original study from 2020 correct?
          24 experiments: 4 varieties * 2 conditions * 3 replicates
          Bioproject: PRJNA611819
          https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA611819

          Show
          robofjoy Robert Reid added a comment - Part 1: Bioproject PRJNA980666 54 samples: 4 varieties * 3 time points * 2 conditions *3 replicates Part 2 Mature Pollen: I think this is the original study from 2020 correct? 24 experiments: 4 varieties * 2 conditions * 3 replicates Bioproject: PRJNA611819 https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA611819
          Hide
          robofjoy Robert Reid added a comment -

          Email I sent to Mark:

          Part 1:Bioproject  PRJNA980666
          54 samples:  4 varieties * 3 time points * 2 conditions *3 replicates

          Part 2 Mature Pollen:I think this is the original study from 2020 correct?24 experiments: 4 varieties * 2 conditions * 3 replicatesBioproject: PRJNA611819https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA611819

          Part 3:ProteomicsThis cannot go into NCBI like the other datasets.There is a org that is dedicated to public repos for proteomic data, https://www.proteomexchange.org/
          This seems to be the front runner.

          The Proteome exchange has 3 venues for data uploading:he GUI based PX Submission Tool can be downloaded to start data upload.PeptideAtlas - PASSEL SRM/MRM data can be submitted to PASSEL.MassIVEShotgun proteomics data can be submitted to MassIVE.
          As to which is best suited, I do not know!

          Show
          robofjoy Robert Reid added a comment - Email I sent to Mark: Part 1:Bioproject  PRJNA980666 54 samples:  4 varieties * 3 time points * 2 conditions *3 replicates Part 2 Mature Pollen:I think this is the original study from 2020 correct?24 experiments: 4 varieties * 2 conditions * 3 replicatesBioproject: PRJNA611819https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA611819 Part 3:ProteomicsThis cannot go into NCBI like the other datasets.There is a org that is dedicated to public repos for proteomic data,  https://www.proteomexchange.org/ This seems to be the front runner. The Proteome exchange has 3 venues for data uploading:he GUI based PX Submission Tool can be downloaded to start data upload.PeptideAtlas - PASSEL SRM/MRM data can be submitted to PASSEL.MassIVEShotgun proteomics data can be submitted to MassIVE. As to which is best suited, I do not know!
          Hide
          robofjoy Robert Reid added a comment -

          Will wait for response from them before closing this ticket.
          We might be submitting proteome data! (which we do not have).

          Show
          robofjoy Robert Reid added a comment - Will wait for response from them before closing this ticket. We might be submitting proteome data! (which we do not have).
          Hide
          robofjoy Robert Reid added a comment -

          Comment from Mark:

          I'm going to ask our proteomics person whether he recommends a specific repository and will let you know what he says.

          Best,

          Mark

          I will close this ticket and in the event they need help with Proteomics data managing, we can open a new ticket.

          Show
          robofjoy Robert Reid added a comment - Comment from Mark: I'm going to ask our proteomics person whether he recommends a specific repository and will let you know what he says. Best, Mark I will close this ticket and in the event they need help with Proteomics data managing, we can open a new ticket.
          robofjoy Robert Reid made changes -
          Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
          robofjoy Robert Reid made changes -
          Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
          robofjoy Robert Reid made changes -
          Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
          robofjoy Robert Reid made changes -
          Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
          robofjoy Robert Reid made changes -
          Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
          robofjoy Robert Reid made changes -
          Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
          robofjoy Robert Reid made changes -
          Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
          robofjoy Robert Reid made changes -
          Resolution Done [ 10000 ]
          Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]

            People

            • Assignee:
              robofjoy Robert Reid
              Reporter:
              robofjoy Robert Reid
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              • Created:
                Updated:
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