Details
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Type:
Task
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Status: Closed (View Workflow)
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Priority:
Major
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Resolution: Done
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Affects Version/s: None
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Fix Version/s: None
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Labels:None
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Story Points:1
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Epic Link:
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Sprint:Summer 1, Summer 2, Summer 3, Summer 4
Description
GOAL: To try a preliminary test run of spades on a Kelsey dataset.
This is a simple pilot run.
From this we can learn some nuances of spades and do a new ticket to do an official run.
Rob will get the URC folks to make a module of the latest SPADES release from earlier this week.
Why Spades?
Trinity creates hundreds of thousands of contigs and is a messy difficult tool.
https://academic.oup.com/gigascience/article/8/9/giz100/5559527
"This article is from 2019, so it may be a little dated. It compared several de novo transcriptome assemblers, such as Trinity and Bloom. Something cool is that it calculates the k-mer size based on read length and it has a lower duplication ratio when compared to trinity or bloom." Brandon
Trinity has a massive duplication problem it seems.