Details
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Type:
Task
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Status: Closed (View Workflow)
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Priority:
Major
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Resolution: Done
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Affects Version/s: None
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Fix Version/s: None
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Labels:None
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Story Points:2
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Sprint:Summer 3, Summer 4
Description
For this task, create RNA-Seq alignments files (BAM), junction files (bed.gz), and scaled coverage graphs (bedgraph.gz) for data set SRP484252, submitted to Sequence Read Archive by Goldstein Lab at UNC Chapel Hill in 2024.
All code will be saved to main branch in this repository - see: https://bitbucket.org/lorainelab/tardigrade/src/main/
Attachments
Issue Links
Activity
Running prefetch jobs with:
cut -d , -f 1 SRP484252_SraRunTable.txt | grep -v Run | xargs -I A sbatch --export=S=A --job-name=A --output=A.out --error=A.err prefetch.sh
in:
/projects/tomato_genome/fnb/dataprocessing/tardigrade/SRP484252/fastq
Confirmed it worked with:
[aloraine@str-i2 fastq]$ cat *.out | grep -c "was downloaded successfully"
12
[aloraine@str-i2 fastq]$ cut -d , -f 1 SRP484252_SraRunTable.txt | grep -v Run | wc -l
12
All 12 runs were prefetched correctly.
Testing:
Opened genome assembly version H_exemplaris_Z151_Apr_2017, opened folder containing SRP484252, opened each read alignment file, coverage graphs, and junction files folder by selecting each checkbox, zooming in, and clicking Load Data.
All files properly loaded!