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  1. IGB
  2. IGBF-3838

Obtain and view sequence read alignments for PBMC dataset from Seurat vignette

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
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      Description

      For this task, locate a BAM format data files for the RNA-Seq data set described in this Seurat tutorial:

      The above tutorial demonstrates features of the Seurat single-cell RNA-Seq data analysis library using data from peripheral blood mononuclear Cells (PBMC) originally from the 10X Genomics Web site.

      Open the data file in IGB. Use the Seurat library (in R) to locate some genes with high counts and then check that IGB shows the same or similar numbers of read alignments.

      Then, make it possible for other people to also open the file and view the contents in IGB.

      Note: If you get stuck on figuring out how to use Seurat for this, ask for help from one of our many local R experts.

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              • Assignee:
                karthik Karthik Raveendran
                Reporter:
                ann.loraine Ann Loraine
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