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  1. IGB
  2. IGBF-4029

Investigate GFF file from NCBI not loading in IGB

    Details

    • Type: Improvement
    • Status: To-Do (View Workflow)
    • Priority: Major
    • Resolution: Unresolved
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      Situation: NCBI has many genomes and annotations that are useful to IGB users. Gene annotations are provided as a GFF or GTF file. The GFF (have not tested GTF) file does not currently load correctly in IGB.

      Task: Investigate why the NCBI GFF/GTF file is not loading correctly in IGB.

      To reproduce the issue:

      1. In a web browser:
      2. Go to https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_000328365.1/
      3. Click Download
      4. Select file types > Genome sequences (FASTA), Annotation features (GTF), and Annotation features (GFF)
      5. Click Download
      6. Unzip the ncbi_dataset.zip file
      7. In IGB:
      8. Click File > Open Genome from File...
      9. Select Reference Sequence > Browse...
      10. Select the GCA_000328365.1_Capca1_genomic.fna file in the unzipped ncbi_dataset folder
      11. Click OK
      12. Select File > Open File...
      13. Select the genomic.gff file in the unzipped ncbi_dataset folder
      14. Click Open
      15. Note the errors in the IGB Log

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              • Assignee:
                Unassigned
                Reporter:
                nfreese Nowlan Freese
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                Dates

                • Created:
                  Updated: