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  1. IGB
  2. IGBF-4107

Blast de novo contigs against 4 contaminants

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      GOAL:

      To write a script on the cluster based on previous scripts to BLAST
      our de novo contigs against the 4 main contaminants found via the FCS filter tool.

      The 4 contaminants are in:
      /projects/tomato_genome/db/contaminants/

      1. sclerotinia_minor
      2. Sclerotinia_sclerotiorum
      3. Pseudocercospora_ulei
      4. Cladosporium_colombiae

      These were pulled from NCBI as full genomes.

      Need to makeblastDB for each contaminant.

        Attachments

          Activity

          robofjoy Robert Reid created issue -
          robofjoy Robert Reid made changes -
          Field Original Value New Value
          Epic Link IGBF-2993 [ 21429 ]
          robofjoy Robert Reid made changes -
          Status To-Do [ 10305 ] In Progress [ 3 ]
          Hide
          bbendick Brandon Bendickson added a comment - - edited

          -rerun at 1e-25
          -get unique query IDs
          -get number of hits
          -make chart with this info

          Show
          bbendick Brandon Bendickson added a comment - - edited -rerun at 1e-25 -get unique query IDs -get number of hits -make chart with this info
          Show
          bbendick Brandon Bendickson added a comment - Storing BLAST information on this sheet: https://docs.google.com/spreadsheets/d/1HeMRLHCuAZsEoXIq4sxLkK8qyuVMRkBzTQBsngx2IdE/edit?usp=sharing
          Hide
          robofjoy Robert Reid added a comment -

          Note: This is related to ticket IGBF-4093, the tomato mold story.

          Show
          robofjoy Robert Reid added a comment - Note: This is related to ticket IGBF-4093 , the tomato mold story.
          robofjoy Robert Reid made changes -
          Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
          robofjoy Robert Reid made changes -
          Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
          robofjoy Robert Reid made changes -
          Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
          robofjoy Robert Reid made changes -
          Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
          robofjoy Robert Reid made changes -
          Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
          robofjoy Robert Reid made changes -
          Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
          robofjoy Robert Reid made changes -
          Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
          robofjoy Robert Reid made changes -
          Resolution Done [ 10000 ]
          Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]

            People

            • Assignee:
              bbendick Brandon Bendickson
              Reporter:
              robofjoy Robert Reid
            • Votes:
              0 Vote for this issue
              Watchers:
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              Dates

              • Created:
                Updated:
                Resolved: