Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      GOAL:
      Explore once again how Deseq2 does its normalization.
      Are there any red flags as to why we cannot use this instead of RPKM, TPM etc.???

      Capture in a Google slide deck in the Kelsey unpol folder.

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          Activity

          robofjoy Robert Reid created issue -
          robofjoy Robert Reid made changes -
          Field Original Value New Value
          Epic Link IGBF-2993 [ 21429 ]
          robofjoy Robert Reid made changes -
          Status To-Do [ 10305 ] In Progress [ 3 ]
          Hide
          robofjoy Robert Reid added a comment -

          https://docs.google.com/presentation/d/1E56wOOJ1xJgwJzF6pT_qXDcIUkFzC5JYvBDphF-wUYQ/edit?usp=sharing

          This is a slide deck that summarizes what DEseq2 does when normalizing and gives explanations why not to use RPKM, etc.

          Show
          robofjoy Robert Reid added a comment - https://docs.google.com/presentation/d/1E56wOOJ1xJgwJzF6pT_qXDcIUkFzC5JYvBDphF-wUYQ/edit?usp=sharing This is a slide deck that summarizes what DEseq2 does when normalizing and gives explanations why not to use RPKM, etc.
          nfreese Nowlan Freese made changes -
          Sprint Summer 1 [ 218 ] Summer 1, Summer 2 [ 218, 219 ]
          nfreese Nowlan Freese made changes -
          Rank Ranked higher
          Hide
          robofjoy Robert Reid added a comment -

          This is done since Brandon found the feature in pathview. So this is done.

          Show
          robofjoy Robert Reid added a comment - This is done since Brandon found the feature in pathview. So this is done.
          robofjoy Robert Reid made changes -
          Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
          robofjoy Robert Reid made changes -
          Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
          robofjoy Robert Reid made changes -
          Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
          robofjoy Robert Reid made changes -
          Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
          robofjoy Robert Reid made changes -
          Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
          robofjoy Robert Reid made changes -
          Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
          robofjoy Robert Reid made changes -
          Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
          robofjoy Robert Reid made changes -
          Resolution Done [ 10000 ]
          Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]

            People

            • Assignee:
              robofjoy Robert Reid
              Reporter:
              robofjoy Robert Reid
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              Dates

              • Created:
                Updated:
                Resolved: