This worked out GREAT !!!!!!
This might be a great case study as to why to use IGB to look at data. Had we done this sooner, it would have saved us a bunch of pointless analyses!
https://docs.google.com/presentation/d/1Jj1xEh6ZZpvSubwsB4kCsFYGVKcBUOjfmRi3Yq7OQdg/edit?usp=sharing
Summary of this:
Basically, we were hunting for mutations in the 3'UTR regions.
We were not finding much.
Upon loading the data into IGB, I was able to pull up the bedgraphs and show that most of the reads are aligning in the gene regions (this technique is Dart-Seq, not Rna-seq).
Few to no reads in the 3'UTR regions!
Mystery solved thanks to IGB.
Case closed.
Hopefully the end of our chapter with Kausik.
I SHOULD add gene annotations to the result. That could be a new ticket.
Task
Major
This worked out GREAT !!!!!!
This might be a great case study as to why to use IGB to look at data. Had we done this sooner, it would have saved us a bunch of pointless analyses!
https://docs.google.com/presentation/d/1Jj1xEh6ZZpvSubwsB4kCsFYGVKcBUOjfmRi3Yq7OQdg/edit?usp=sharing
Summary of this:
Basically, we were hunting for mutations in the 3'UTR regions.
We were not finding much.
Upon loading the data into IGB, I was able to pull up the bedgraphs and show that most of the reads are aligning in the gene regions (this technique is Dart-Seq, not Rna-seq).
Few to no reads in the 3'UTR regions!
Mystery solved thanks to IGB.
Case closed.
Hopefully the end of our chapter with Kausik.
I SHOULD add gene annotations to the result. That could be a new ticket.