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  1. Bugs That Are No Longer Relevant
  2. OLD-77

Bam Read packing issue: Selected read (identified by sequence) doesn't appear in sliced view because the reads pack differently in the sliced view

    Details

    • Type: Bug
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Fixed
    • Labels:
      None

      Description

      Open a BAM file; do not load sequence. Open sliced view. Zoom in until you can see the actual sequence on the reads. Double click/select a read that is in a relatively deep stack (>20 reads) but set the read depth to 10. There is a very good chance that the selected read does NOT appear in the sliced view because it gets incorporated into the summary/collapsed line of reads that the top of the frame. Also notice that the reads showing in the zoomed in main view are NOT the same set of reads being shown in the sliced view - all of this is due to read packing.

      Sourceforge# 3046549

      **editted to refine the problem (original issue was that the sequence of a selected read is not appearing in the sliced view.)

        Attachments

          Activity

          afshan Afshan Jalali (Inactive) created issue -
          afshan Afshan Jalali (Inactive) made changes -
          Field Original Value New Value
          Status Open [ 1 ] Closed [ 6 ]
          Resolution Fixed [ 1 ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Project IGB6.5 [ 10010 ] Bug Repository [ 10030 ]
          Key IGB-70 BUG-43
          alyssa Alyssa Gulledge (Inactive) made changes -
          Resolution Fixed [ 1 ]
          Status Closed [ 6 ] Reopened [ 4 ]
          david David Norris (Inactive) made changes -
          Assignee Afshan Jalali [ afshan ]
          david David Norris (Inactive) made changes -
          Status Reopened [ 4 ] Closed [ 6 ]
          Resolution Fixed [ 1 ]
          david David Norris (Inactive) made changes -
          Resolution Fixed [ 1 ]
          Status Closed [ 6 ] Reopened [ 4 ]
          david David Norris (Inactive) made changes -
          Status Reopened [ 4 ] Resolved [ 5 ]
          Resolution Fixed [ 1 ]
          david David Norris (Inactive) made changes -
          Status Resolved [ 5 ] Closed [ 6 ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Resolution Fixed [ 1 ]
          Status Closed [ 6 ] Reopened [ 4 ]
          Assignee Katharine Kubiak [ kkubiak ]
          kkubiak Katharine Kubiak (Inactive) made changes -
          Assignee Katharine Kubiak [ kkubiak ] Alyssa Gulledge [ alyssa ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Summary Read sequences don't appear in sliced view Bam Read packing issue: Selected read (identified by sequence) doesn't appear in sliced view because the reads pack differently in the sliced view
          Description 1. Open IGB; Arabidopsis thaliana, Jun_2009

          2. Load a sample BAM file from http://www.bioviz.org/quickload/A_thaliana_Jun_2009/nextgen_samples/

          3. Set the horizontal zoom to the far right BEFORE loading the BAM file.

          4. Character sequences can be seen in the view.

          5. Click on the Sliced view.

          6. The character sequences should appear in the "sliced view" also, as they appear in the upper view.

          Sourceforge# 3046549

          Testing Results:
          Sept 30, 2010 - Alyssa Gulledge

          version 6.4 (6969) This bug is still present. The sliced view does NOT show the GATC sequence that can be seen in the upper window.
          Open a BAM file; do not load sequence. Open sliced view. Zoom in until you can see the actual sequence on the reads. Double click/select a read that is in a relatively deep stack (>20 reads) but set the read depth to 10. There is a very good chance that the selected read does NOT appear in the sliced view because it gets incorporated into the summary/collapsed line of reads that the top of the frame. Also notice that the reads showing in the zoomed in main view are NOT the same set of reads being shown in the sliced view - all of this is due to read packing.

          Sourceforge# 3046549

          **editted to refine the problem
          alyssa Alyssa Gulledge (Inactive) made changes -
          Description Open a BAM file; do not load sequence. Open sliced view. Zoom in until you can see the actual sequence on the reads. Double click/select a read that is in a relatively deep stack (>20 reads) but set the read depth to 10. There is a very good chance that the selected read does NOT appear in the sliced view because it gets incorporated into the summary/collapsed line of reads that the top of the frame. Also notice that the reads showing in the zoomed in main view are NOT the same set of reads being shown in the sliced view - all of this is due to read packing.

          Sourceforge# 3046549

          **editted to refine the problem
          Open a BAM file; do not load sequence. Open sliced view. Zoom in until you can see the actual sequence on the reads. Double click/select a read that is in a relatively deep stack (>20 reads) but set the read depth to 10. There is a very good chance that the selected read does NOT appear in the sliced view because it gets incorporated into the summary/collapsed line of reads that the top of the frame. Also notice that the reads showing in the zoomed in main view are NOT the same set of reads being shown in the sliced view - all of this is due to read packing.

          Sourceforge# 3046549

          **editted to refine the problem (original issue was that the sequence of a selected read is not appearing in the sliced view.)
          alyssa Alyssa Gulledge (Inactive) made changes -
          Assignee Alyssa Gulledge [ alyssa ] Hiral Vora [ hiralv ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Project Bug Repository [ 10030 ] IGB 6.8 [ 10140 ]
          Key BUG-43 IGBC-38
          hiralv Hiral Vora (Inactive) made changes -
          Assignee Hiral Vora [ hiralv ]
          hiralv Hiral Vora (Inactive) made changes -
          Assignee Alyssa Gulledge [ alyssa ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Assignee Alyssa Gulledge [ alyssa ] Katharine Kubiak [ kkubiak ]
          kkubiak Katharine Kubiak (Inactive) made changes -
          Status Reopened [ 4 ] Resolved [ 5 ]
          Assignee Katharine Kubiak [ kkubiak ]
          Resolution Fixed [ 1 ]
          kkubiak Katharine Kubiak (Inactive) made changes -
          Project IGB 7.0 [ 10140 ] Bug Repository [ 10030 ]
          Key IGBC-38 BUG-466
          Reporter Afshan Jalali [ afshan ] Katharine Kubiak [ kkubiak ]
          richard Richard Linchangco (Inactive) made changes -
          Resolution Fixed [ 1 ]
          Status Resolved [ 5 ] Reopened [ 4 ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Link This issue relates to BUG-661 [ BUG-661 ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Link This issue relates to BUG-661 [ BUG-661 ]
          alyssa Alyssa Gulledge (Inactive) made changes -
          Project Bug Repository [ 10030 ] Bugs That Are No Longer Relevant [ 10041 ]
          Key BUG-466 OLD-77
          Reporter Katharine Kubiak [ kkubiak ] Alyssa Gulledge [ alyssa ]
          hiralv Hiral Vora (Inactive) made changes -
          Status Reopened [ 4 ] Closed [ 6 ]
          Resolution Fixed [ 1 ]

            People

            • Assignee:
              Unassigned
              Reporter:
              alyssa Alyssa Gulledge (Inactive)
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              Dates

              • Created:
                Updated:
                Resolved: