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  1. Testing
  2. TEST-22

Test http://deeptools.ie-freiburg.mpg.de/ BAM tools

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    • Type: Task
    • Status: Open (View Workflow)
    • Priority: Major
    • Resolution: Unresolved
    • Affects Version/s: None
    • Fix Version/s: None
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      Description

      This Galaxy mirror:

      http://deeptools.ie-freiburg.mpg.de

      implements a number of tools for working with BAM files.

      I'd like you to test whether or not their mirror enables IGB visualization of BAM and bigwig files.

      To test, use my account:

      user name: Aloraine@uncc.edu (note it is case sensitive)
      password: (ask me)

      I've upload a BAM file for Arabidopsis.

      First, make an index for it using samtools index.

      Next, run the bamCoverage tool on it, which outputs a bigwig file. To get the genome size, add up the values in genome.txt for Arabidopsis on the IGB Quickload site. You can do it very easily in R - just read in the genome.txt file from the URL and calculate the sum.

      Make an issue for this in Jira and link it to the bigwig bug issue I reported.

      Thanks and if you have any questions please let me know!!!

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            • Assignee:
              ann.loraine Ann Loraine
              Reporter:
              kyle Kyle Suttlemyre (Inactive)
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              • Created:
                Updated: