# get general resource
wget http://igbquickload.org/A_thaliana_Jun_2009/genome.txt


# To create bigbed file
# bash
wget http://lorainelab-quickload.scidas.org/rnaseq/A_thaliana_Jun_2009/SRP022162/Pollen.bed.gz

# R
p = read.delim("Pollen.bed", header=F)
p$V5=0
write.table(p, "Pollen.noScore.bed", sep="\t", col.names=F, row.names=F, quote=F)

# bash
bedToBigBed Pollen.noScore.bed genome.txt PollenJuncs.noScore.bb


# to create bigwig file
wget http://lorainelab-quickload.scidas.org/rnaseq/A_thaliana_Jun_2009/SRP022162/Pollen.bedgraph.gz
wigToBigWig Pollen.bedgraph genomeC.txt Pollen.bw

# to create wig
bigWigToWig Pollen.bw Pollen.wig