Open Sequence Viewer (Whole Gene Model)

• View Menu
• Shortcut keystroke
• Toolbar button (First enable, then use)
• Right-click Menu

Open items in Sequence Viewer:

• Ensure title bar accuracy
• 'Show cDNA' grayed out except for gene models
• Whole gene model
• Single exon (CDS and UTR)
• Highlighted/Selected sequence
• Cytoband (Human or Mouse)

View Short reads (Load a BAM file):

• Open Short read sequence via View, keystroke, Toolbar button, and right-click Menu (Known issue:Selection lost with right-click Menu)
• Short Read, Genomic
• Short Read, Insertion (Insertion different color) and Deletion

File (Open saved file to check accuracy):

• Save As Fasta
• Save As Fasta (Reverse Complement)
• Export Image
• Close Sequence Viewer (Menu and Keystroke)

Copy (Paste into Text Editor to ensure accuracy):

• Select All (Menu and Keystroke)
• Copy Selected DNA Sequence
• Copy DNA with lowercase...
• Copy Annotated Translation
• Copy Selected Translation(s)

Show

• Reverse Complement (Ensure start and stop are accurately identified)
• Complement
• All + Translation
• All - Translation

Colors/'Show cDNA' (Use Whole Gene Model):

• Check both color schemes
• Ensure 'Show cDNA' and 'Show genomic' work (Button switches AND function works)