From: Mason Meyer <mmeyer20@uncc.edu>
To: Rajesh Biswas <biswasr0@gmail.com>
CC: "Loraine, Ann" <Ann.Loraine@uncc.edu>
Date: Wed, Sep 20, 2017 at 10:26 AM
Subject: Re: view read sequence
Hello Rajesh,
My name is Mason Meyer, IGB Support Specialist. Thank you for contacting us with your question. After viewing the screenshots you sent, it appears to me that your files are BED files and the "View Read Sequence" feature will only work for BAM files containing read alignments.
For your case, the "View Genomic Sequence" option should allow you to view the selected peak's sequence. If that allows you to accomplish your goal, please let me know and I will mark your issue as resolved, but if not, please don't hesitate to contact me and I will continue to look into the issue. Also, if you have any additional feedback for our team, we would love to hear it!
Thanks again,
Mason Meyer
IGB Support Specialist
From: Mason Meyer <mmeyer20@uncc.edu>
To: Rajesh Biswas <biswasr0@gmail.com>
Date: Fri, Sep 22, 2017 at 12:31 PM
Subject: Re: view read sequence
Hello again Rajesh,
Unfortunately, I don't believe you will be able to view the sequence using a BED file, since no sequence data will be contained in it. It is possible that the program that output your BED file used one of the BED file's columns to include the sequence data, but I have personally never seen this. If you would like to share your file with me, I would be happy to check it out for you and tell you if it contains any sequence data, but it most likely does not. You could also open the file in a text editor and if you see what looks like sequence data, let me know and I can assist you in viewing this info in IGB by viewing the peak's selection info (this info is also contained in the tooltip when you hover on an annotation in IGB).
If you have any other questions or feedback, please don't hesitate to contact me.
Sincerely,
Mason Meyer
IGB Support Specialist