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  1. IGB
  2. IGBF-1266

Create a bigbed file for Galaxy testing

    Details

    • Type: Bug
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      Find or create a bigbed file in the A. thaliana June 2009 genome.
      Send it to Ann to add to the Galaxy files for testing purposes.

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            ieclabau Ivory Blakley (Inactive) created issue -
            ieclabau Ivory Blakley (Inactive) made changes -
            Field Original Value New Value
            Link This issue relates to IGBF-1246 [ IGBF-1246 ]
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            The Galaxy history (https://usegalaxy.org/u/aloraine/h/cold-stress-fastq) listed in the release testing instructions (https://wiki.transvar.org/display/ITD/Galaxy) does not include a wig or bigwig file.

            See if Ann would also needs those.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - The Galaxy history ( https://usegalaxy.org/u/aloraine/h/cold-stress-fastq ) listed in the release testing instructions ( https://wiki.transvar.org/display/ITD/Galaxy ) does not include a wig or bigwig file. See if Ann would also needs those.
            ieclabau Ivory Blakley (Inactive) made changes -
            Status Open [ 1 ] In Progress [ 3 ]
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            Yes, we need all three. Send them to Ann when they are ready.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - Yes, we need all three. Send them to Ann when they are ready.
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            We have each of those file types in the human genome, see smoke testing quickload:
            http://igbquickload.org/smokeTestingQuickload/H_sapiens_Dec_2013/

            The Galaxy test set is all in Arabidopsis, but ... does it have to be all in one genome?
            --upside to making A. thaliana files: we have multiple complete test sets.
            --downside of making A. thaliana files: takes effort, not sure exactly how much.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - We have each of those file types in the human genome, see smoke testing quickload: http://igbquickload.org/smokeTestingQuickload/H_sapiens_Dec_2013/ The Galaxy test set is all in Arabidopsis, but ... does it have to be all in one genome? --upside to making A. thaliana files: we have multiple complete test sets. --downside of making A. thaliana files: takes effort, not sure exactly how much.
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            BigBed:
            Use bedToBigBed from blat suite, see https://genome.ucsc.edu/goldenpath/help/bigBed.html

            BigWig:
            Use wigToBigWig from blat suite, see https://genome.ucsc.edu/goldenpath/help/bigWig.html

            Wig:
            Use bigWigToWig from blat suite, see help page.

            Need to pick out some bed file to use for the initial input....

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - BigBed: Use bedToBigBed from blat suite, see https://genome.ucsc.edu/goldenpath/help/bigBed.html BigWig: Use wigToBigWig from blat suite, see https://genome.ucsc.edu/goldenpath/help/bigWig.html Wig: Use bigWigToWig from blat suite, see help page. Need to pick out some bed file to use for the initial input....
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            The files are in a google drive folder which I have shared with Ann.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - The files are in a google drive folder which I have shared with Ann.
            ieclabau Ivory Blakley (Inactive) made changes -
            Attachment HowToMakeFiles.txt [ 14081 ]
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment - - edited

            I attached the file of instructions to make the three file formats. Requires the blat suite.

            I ran into some difficulty with making a bigbed file from the TAIR10.bed file. The bedToBigBed converter complained about something in the description field(s). After I removed those fields, the converter made a bigbed file, but IGB would not display the data (just loaded empty space). I created issue IGBF-1272 to address this. Rather than convert all of the chromosome names, I picked a different started file that used names to match the A. thaliana genome: Pollen.bed. The bedToBigBed converter complained about scores that were greater than 1000, so I added a step to set all scores to 0. With this, IGB will load the file and display features. But each feature is shown as a single block (original file was junctions--each feature is two blocks), and the strand info seems to have been lost. I created issue IGBF-1273 to investigate this.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - - edited I attached the file of instructions to make the three file formats. Requires the blat suite. I ran into some difficulty with making a bigbed file from the TAIR10.bed file. The bedToBigBed converter complained about something in the description field(s). After I removed those fields, the converter made a bigbed file, but IGB would not display the data (just loaded empty space). I created issue IGBF-1272 to address this. Rather than convert all of the chromosome names, I picked a different started file that used names to match the A. thaliana genome: Pollen.bed. The bedToBigBed converter complained about scores that were greater than 1000, so I added a step to set all scores to 0. With this, IGB will load the file and display features. But each feature is shown as a single block (original file was junctions--each feature is two blocks), and the strand info seems to have been lost. I created issue IGBF-1273 to investigate this.
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            I am reassigning to Ann to add these files to the Galaxy history for testing.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - I am reassigning to Ann to add these files to the Galaxy history for testing.
            ieclabau Ivory Blakley (Inactive) made changes -
            Assignee Ivory Blakley [ ieclabau ] Ann Loraine [ aloraine ]
            ieclabau Ivory Blakley (Inactive) made changes -
            Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
            Hide
            ann.loraine Ann Loraine added a comment -

            uploaded to public Galaxy History "Open Galaxy files in Integrated Genome Browser"

            Show
            ann.loraine Ann Loraine added a comment - uploaded to public Galaxy History "Open Galaxy files in Integrated Genome Browser"
            ann.loraine Ann Loraine made changes -
            Comment [ uploaded to Galaxy History "Open Galaxy files in Integrated Genome Browser" ]
            ann.loraine Ann Loraine made changes -
            Assignee Ann Loraine [ aloraine ] Ivory Blakley [ ieclabau ]
            ieclabau Ivory Blakley (Inactive) made changes -
            Status Needs 1st Level Review [ 10005 ] Reviewing [ 10301 ]
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            For two of the files, the file genome ("database") is set to "Arabidopsis_thaliana_TAIR10", and they appear in IGB in the Arabidopsis genome.

            The wig file is set to "arabidopsis", so it appears in IGB in a custom genome, apart from any other data.

            I am reassigning to Ann to correct the genome attribute of the wig file.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - For two of the files, the file genome ("database") is set to "Arabidopsis_thaliana_TAIR10", and they appear in IGB in the Arabidopsis genome. The wig file is set to "arabidopsis", so it appears in IGB in a custom genome, apart from any other data. I am reassigning to Ann to correct the genome attribute of the wig file.
            ieclabau Ivory Blakley (Inactive) made changes -
            Assignee Ivory Blakley [ ieclabau ] Ann Loraine [ aloraine ]
            ieclabau Ivory Blakley (Inactive) made changes -
            Status Reviewing [ 10301 ] Needs 1st Level Review [ 10005 ]
            Hide
            ann.loraine Ann Loraine added a comment -

            All files now have Galaxy genome version set to "Arabidopsis thaliana TAIR10"

            Show
            ann.loraine Ann Loraine added a comment - All files now have Galaxy genome version set to "Arabidopsis thaliana TAIR10"
            ann.loraine Ann Loraine made changes -
            Assignee Ann Loraine [ aloraine ] Ivory Blakley [ ieclabau ]
            ann.loraine Ann Loraine made changes -
            Status Needs 1st Level Review [ 10005 ] Ready for Pull Request [ 10304 ]
            ann.loraine Ann Loraine made changes -
            Status Ready for Pull Request [ 10304 ] In Progress [ 3 ]
            Hide
            ieclabau Ivory Blakley (Inactive) added a comment -

            The wig files shows up in the right genome now.
            All files appear as they do when I load them from my local machine.

            I am marking this issue as complete.

            Show
            ieclabau Ivory Blakley (Inactive) added a comment - The wig files shows up in the right genome now. All files appear as they do when I load them from my local machine. I am marking this issue as complete.
            ieclabau Ivory Blakley (Inactive) made changes -
            Resolution Done [ 10000 ]
            Status In Progress [ 3 ] Closed [ 6 ]
            ann.loraine Ann Loraine made changes -
            Workflow Loraine Lab Workflow [ 17957 ] Fall 2019 Workflow Update [ 19873 ]
            ann.loraine Ann Loraine made changes -
            Workflow Fall 2019 Workflow Update [ 19873 ] Revised Fall 2019 Workflow Update [ 21992 ]
            ann.loraine Ann Loraine made changes -
            Epic Link IGBF-1880 [ 17970 ]
            ann.loraine Ann Loraine made changes -
            Story Points 1

              People

              • Assignee:
                ieclabau Ivory Blakley (Inactive)
                Reporter:
                ieclabau Ivory Blakley (Inactive)
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                Dates

                • Created:
                  Updated:
                  Resolved: