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  1. IGB
  2. IGBF-3162

Generate scaled coverage graphs for RNA-Seq alignments

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
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      Description

      Make scaled coverages graphs for the alternative splicing project BAM files.

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            ann.loraine Ann Loraine added a comment - - edited

            Added scripts to new repository hotpollen/splicing-analysis.
            Created new directory:

            /nobackup/tomato_genome/alt_splicing/coverage_graph_bams
            

            in UNCC cluster with symbolic links to bam files made in IGBF-3143 and IGBF-3163.

            Made symbolic links to code in my home directory for generating coverage graphs. Launched jobs with:

            sbatch-doIt.sh .bam bamCoverage.sh >jobs.out 2>jobs.err
            

            Seems to be running OK.

            Scripts used:

            • src/sbatch-doIt.sh
            • src/bamCoverage.sh

            in repository https://bitbucket.org/hotpollen/splicing-analysis

            Show
            ann.loraine Ann Loraine added a comment - - edited Added scripts to new repository hotpollen/splicing-analysis. Created new directory: /nobackup/tomato_genome/alt_splicing/coverage_graph_bams in UNCC cluster with symbolic links to bam files made in IGBF-3143 and IGBF-3163 . Made symbolic links to code in my home directory for generating coverage graphs. Launched jobs with: sbatch-doIt.sh .bam bamCoverage.sh >jobs.out 2>jobs.err Seems to be running OK. Scripts used: src/sbatch-doIt.sh src/bamCoverage.sh in repository https://bitbucket.org/hotpollen/splicing-analysis
            Hide
            ann.loraine Ann Loraine added a comment - - edited

            Files are done.

            I moved them to /nobackup/tomato_genome/alt_splicing/for_igbquickload/coverage_graphs.

            To review:

            • check that files have reasonable sizes (nothing non-zero)
            • check that every ".scaled.bedgraph.gz" file has a corresponding ".scaled.bedgraph.gz.tbi" index file
            • check that every "SRR" bam file in our control and experimental sample directories has a corresponding "scaled.bedgraph.gz" file
            Show
            ann.loraine Ann Loraine added a comment - - edited Files are done. I moved them to /nobackup/tomato_genome/alt_splicing/for_igbquickload/coverage_graphs. To review: check that files have reasonable sizes (nothing non-zero) check that every ".scaled.bedgraph.gz" file has a corresponding ".scaled.bedgraph.gz.tbi" index file check that every "SRR" bam file in our control and experimental sample directories has a corresponding "scaled.bedgraph.gz" file
            Hide
            Mdavis4290 Molly Davis added a comment - - edited

            Review:

            Directory- /nobackup/tomato_genome/alt_splicing/for_igbquickload/coverage_graphs

            • No files seemed to have zero file size. Used command LL/ll.
            • Every ".scaled.bedgraph.gz" file has a ".scaled.bedgraph.gz.tbi" file.
            • Every "SRR" bam file in our control and experimental sample directories has a corresponding "scaled.bedgraph.gz" file. SRP328042-molly SRR files are in-between SRP252265 files in the directory might be hard to differentiate which is control and which is experimental.

            [~aloraine]

            Show
            Mdavis4290 Molly Davis added a comment - - edited Review: Directory- /nobackup/tomato_genome/alt_splicing/for_igbquickload/coverage_graphs No files seemed to have zero file size. Used command LL/ll. Every ".scaled.bedgraph.gz" file has a ".scaled.bedgraph.gz.tbi" file. Every "SRR" bam file in our control and experimental sample directories has a corresponding "scaled.bedgraph.gz" file. SRP328042-molly SRR files are in-between SRP252265 files in the directory might be hard to differentiate which is control and which is experimental. [~aloraine]
            Hide
            ann.loraine Ann Loraine added a comment -

            Thanks [~molly]. I decided to store all the files in the same folder and will use an index / table of contents type strategy to distinguish them. Moving to Done.

            Show
            ann.loraine Ann Loraine added a comment - Thanks [~molly] . I decided to store all the files in the same folder and will use an index / table of contents type strategy to distinguish them. Moving to Done.

              People

              • Assignee:
                ann.loraine Ann Loraine
                Reporter:
                ann.loraine Ann Loraine
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                • Created:
                  Updated:
                  Resolved: