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  1. IGB
  2. IGBF-3434

Identify the aligned region and visualize it in IGB

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
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      Description

      Relates to error: IGBF-3420

      Error:

      Exception in thread "main" java.lang.RuntimeException: Postion is too high (more than 64792705)
      at org.biojava.nbio.genome.parsers.twobit.TwoBitParser.setCurrentSequencePosition(TwoBitParser.java:191)
      at org.biojava.nbio.genome.parsers.twobit.TwoBitParser.loadFragment(TwoBitParser.java:332)
      at org.lorainelab.findjunctions.FindJunctions.main(FindJunctions.java:249)
      

      Issue: Looked at the bam file which looks fine but there are reads that expand upon the chromosome. They have been soft clipped but find_junctions isn't taking that into consideration and won't process the file entirely to create the .FJ.bed.gz file.

      Visualize the error in IGB.

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              • Assignee:
                Mdavis4290 Molly Davis
                Reporter:
                Mdavis4290 Molly Davis
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                • Created:
                  Updated:
                  Resolved: