Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      The goal is to add to the existing EB-SEQ table results (From IBGF-3454).

      The table currently includes the EB-SEQ results along with the SolyID and gene description.

      What we can add:

      • The read expression counts data from Salmon.
      • The deseq2 results, values, log fold changes, pvalues, etc.
      • EdgeR data

      This would produce an unwieldy table, 100's of columns long!
      I am going to first touch base with Gloria about what would be the most useful.

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            robofjoy Robert Reid created issue -
            robofjoy Robert Reid made changes -
            Field Original Value New Value
            Epic Link IGBF-2993 [ 21429 ]
            robofjoy Robert Reid made changes -
            Description
            The goal is to add to the existing EB-SEQ table results (From IBGF-3454).

            The table currently includes the EB-SEQ results along with the SolyID and gene description.

            What we can add:
            * The read expression counts data from Salmon.
            * The deseq2 results, values, log fold changes, pvalues, etc.
            * EdgeR data

            This would produce an unwieldy table, 100's of columns long!
            I am going to first touch base with Gloria about what would be the most useful.
            The goal is to add to the existing EB-SEQ table results (From IBGF-3454).

            The table currently includes the EB-SEQ results along with the SolyID and gene description.

            What we can add:
            * The read expression counts data from Salmon.
            * The deseq2 results, values, log fold changes, pvalues, etc.
            * EdgeR data

            This would produce an unwieldy table, 100's of columns long!
            I am going to first touch base with Gloria about what would be the most useful.
            Sprint Fall 4 2023 Oct 16 [ 180 ]
            Hide
            robofjoy Robert Reid added a comment -

            Did not get to chat with Gloria in Wake Forest. She was juggling 50 things!

            What might look cool in this table would be the MvW results that Ann produced comparing are versus VF36 and recently shared with Gloria's lab.

            Table I refer to is in bitbucket here:
            https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/results/MvW-SL4.txt

            We have the following comparisons:

            awk '

            { print $2 "\t" $3 }

            ' MvW-SL4.txt | sort | uniq

            • A.28 V.28
            • V.28.15 A.28.15
            • V.28.30 A.28.30
            • V.28.45 A.28.45
            • V.28.75 A.28.75
            • V.34.15 A.34.15
            • V.34.30 A.34.30
            • V.34.45 A.34.45
            • V.34.75 A.34.75

            Each of these sets could be added as columns to the EBSEQ table. I am thinking of adding just the LOGFOLD change result for each set.
            Or maybe the P-value also. This would be 9 expts X 2 results for 18 more columns!

            Still manageable I think.
            Sounds like a Python script!

            Show
            robofjoy Robert Reid added a comment - Did not get to chat with Gloria in Wake Forest. She was juggling 50 things! What might look cool in this table would be the MvW results that Ann produced comparing are versus VF36 and recently shared with Gloria's lab. Table I refer to is in bitbucket here: https://bitbucket.org/hotpollen/flavonoid-rnaseq/src/main/72_F3H_PollenTube/results/MvW-SL4.txt We have the following comparisons: awk ' { print $2 "\t" $3 } ' MvW-SL4.txt | sort | uniq A.28 V.28 V.28.15 A.28.15 V.28.30 A.28.30 V.28.45 A.28.45 V.28.75 A.28.75 V.34.15 A.34.15 V.34.30 A.34.30 V.34.45 A.34.45 V.34.75 A.34.75 Each of these sets could be added as columns to the EBSEQ table. I am thinking of adding just the LOGFOLD change result for each set. Or maybe the P-value also. This would be 9 expts X 2 results for 18 more columns! Still manageable I think. Sounds like a Python script!
            ann.loraine Ann Loraine made changes -
            Sprint Fall 4 2023 Oct 16 [ 180 ] Fall 4 2023 Oct 16, Fall 5 [ 180, 181 ]
            ann.loraine Ann Loraine made changes -
            Rank Ranked higher
            robofjoy Robert Reid made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            robofjoy Robert Reid made changes -
            Status In Progress [ 3 ] To-Do [ 10305 ]
            robofjoy Robert Reid made changes -
            Link This issue is blocked by IGBF-3489 [ IGBF-3489 ]
            ann.loraine Ann Loraine made changes -
            Epic Link IGBF-2993 [ 21429 ] IGBF-3446 [ 22548 ]
            robofjoy Robert Reid made changes -
            Status To-Do [ 10305 ] In Progress [ 3 ]
            robofjoy Robert Reid made changes -
            Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
            robofjoy Robert Reid made changes -
            Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
            robofjoy Robert Reid made changes -
            Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
            robofjoy Robert Reid made changes -
            Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
            robofjoy Robert Reid made changes -
            Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
            robofjoy Robert Reid made changes -
            Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
            robofjoy Robert Reid made changes -
            Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
            robofjoy Robert Reid made changes -
            Resolution Done [ 10000 ]
            Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]
            Hide
            robofjoy Robert Reid added a comment -

            Until we hear back from Gloria's group that they plan to harness this analysis in some way, we will close this task.

            When they ask for this again along with more related data, we can open a new ticket at that time.

            Show
            robofjoy Robert Reid added a comment - Until we hear back from Gloria's group that they plan to harness this analysis in some way, we will close this task. When they ask for this again along with more related data, we can open a new ticket at that time.

              People

              • Assignee:
                robofjoy Robert Reid
                Reporter:
                robofjoy Robert Reid
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                  Updated:
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