Uploaded image for project: 'IGB'
  1. IGB
  2. IGBF-3619

Kelsey's quest for unbiased gene distribution

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Minor
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      This task is more of a brainstorm based on the details that came out of Kelsey's committee meeting.
      In her meeting, an advisor inquired about how Heinz is the reference genome and a sample in the experiment. It would make sense that Heinz will more easily align to reference genome compared to the other 3 TMH varieties.

      The PCA (attached) reflects this.

      The goal would be to come up with an unbiased way to check MDS / PCA / PCoA where everything is not aligned to Heinz.

      1 idea.

      1. We de novo assembly via trinity.
      2. Get a set of genes for each variety.
      3. Assign all of these genes to a SolyID via blast. Along with a gene expression count.
      4. Run these as a MDS / PCA and see the distribution.

        Attachments

          Activity

            People

            • Assignee:
              robofjoy Robert Reid
              Reporter:
              robofjoy Robert Reid
            • Votes:
              0 Vote for this issue
              Watchers:
              2 Start watching this issue

              Dates

              • Created:
                Updated:
                Resolved: