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  1. IGB
  2. IGBF-3767

RNA-SPADE2: Testing this tool to replace Trinity

    Details

    • Type: Task
    • Status: Closed (View Workflow)
    • Priority: Major
    • Resolution: Done
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None

      Description

      GOAL: To try a preliminary test run of spades on a Kelsey dataset.

      This is a simple pilot run.

      From this we can learn some nuances of spades and do a new ticket to do an official run.
      Rob will get the URC folks to make a module of the latest SPADES release from earlier this week.

      Why Spades?
      Trinity creates hundreds of thousands of contigs and is a messy difficult tool.

      https://academic.oup.com/gigascience/article/8/9/giz100/5559527

      "This article is from 2019, so it may be a little dated. It compared several de novo transcriptome assemblers, such as Trinity and Bloom. Something cool is that it calculates the k-mer size based on read length and it has a lower duplication ratio when compared to trinity or bloom." Brandon

      Trinity has a massive duplication problem it seems.

        Attachments

          Activity

          robofjoy Robert Reid created issue -
          robofjoy Robert Reid made changes -
          Field Original Value New Value
          Epic Link IGBF-2993 [ 21429 ]
          robofjoy Robert Reid made changes -
          Status To-Do [ 10305 ] In Progress [ 3 ]
          ann.loraine Ann Loraine made changes -
          Sprint Summer 1 [ 195 ] Summer 1, Summer 2 [ 195, 196 ]
          ann.loraine Ann Loraine made changes -
          Rank Ranked higher
          ann.loraine Ann Loraine made changes -
          Sprint Summer 1, Summer 2 [ 195, 196 ] Summer 1, Summer 2, Summer 3 [ 195, 196, 197 ]
          ann.loraine Ann Loraine made changes -
          Rank Ranked higher
          ann.loraine Ann Loraine made changes -
          Sprint Summer 1, Summer 2, Summer 3 [ 195, 196, 197 ] Summer 1, Summer 2, Summer 3, Summer 4 [ 195, 196, 197, 198 ]
          ann.loraine Ann Loraine made changes -
          Rank Ranked higher
          bbendick Brandon Bendickson made changes -
          Status In Progress [ 3 ] Needs 1st Level Review [ 10005 ]
          bbendick Brandon Bendickson made changes -
          Status Needs 1st Level Review [ 10005 ] First Level Review in Progress [ 10301 ]
          bbendick Brandon Bendickson made changes -
          Status First Level Review in Progress [ 10301 ] Ready for Pull Request [ 10304 ]
          bbendick Brandon Bendickson made changes -
          Status Ready for Pull Request [ 10304 ] Pull Request Submitted [ 10101 ]
          bbendick Brandon Bendickson made changes -
          Status Pull Request Submitted [ 10101 ] Reviewing Pull Request [ 10303 ]
          bbendick Brandon Bendickson made changes -
          Status Reviewing Pull Request [ 10303 ] Merged Needs Testing [ 10002 ]
          bbendick Brandon Bendickson made changes -
          Status Merged Needs Testing [ 10002 ] Post-merge Testing In Progress [ 10003 ]
          bbendick Brandon Bendickson made changes -
          Resolution Done [ 10000 ]
          Status Post-merge Testing In Progress [ 10003 ] Closed [ 6 ]

            People

            • Assignee:
              bbendick Brandon Bendickson
              Reporter:
              robofjoy Robert Reid
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              Dates

              • Created:
                Updated:
                Resolved: