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  1. IGB
  2. IGBF-4312

Use Deseq results to define Up-Down patterns in ManuSeq

    Details

    • Type: Task
    • Status: To-Do (View Workflow)
    • Priority: Major
    • Resolution: Unresolved
    • Affects Version/s: None
    • Fix Version/s: None
    • Labels:
      None
    • Story Points:
      3
    • Sprint:
      Fall 2, Fall 3, Fall 4, Fall 5, Fall 6, Fall 7, Fall 8, Fall 9, Spring 1, Spring 2, Spring 3

      Description

      GOAL: We tweak our UP-Down patterns so that we are actually using deseq's results to define whether a gene goes up or down.

      No longer using just the general pattern derived from the normalized data. We goo a few steps furhter in the Deseq script and get the results.

      I predict this will produce wayyyy less up and down patterns and way more even-even type of responses.

      We will need to compare the overall #'s of how many Up-down, up-up, etc we get this way compared to the "version 1" method. (manuseq)

      We will call this way version 2 until we come up with a cooler name. (Pattern de seq)

      Also need to refresh our brains on how Deseq2 defines its cutoffs for significance. We can play with that. We can be less stringent so we get more "ups and downs".

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            • Assignee:
              bbendick Brandon Bendickson
              Reporter:
              robofjoy Robert Reid
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              • Created:
                Updated: